Neuron-glia interaction during cerebellar development The role of Ca-permeable AMPA receptors

小脑Bergmann神经胶质细胞具有钙离子可渗透的AMPA受体，并可通过钙离子介导的神经元-胶质细胞相互作用参与神经回路的形成和发育。为了阐明钙渗透性AMPA受体在小脑神经胶质细胞中的功能作用，我试图开发一种方法，其中胶质细胞钙渗透性AMPA受体在小脑发育过程中变成钙不渗透性。使用截短的GFAP启动子的慢病毒载体，我成功地在神经胶质细胞中有效表达GluR 2基因，向性没有变化。

Cerebellar Bergmann glial cells have Ca-permeable AMPA receptors and can be involved in formation and development of neuronal circuit via neuron-glia interactions mediated by Ca. To elucidate the functional role of Ca-permeable AMPA receptors in cerebellar glial cells, I attempted to develop a method in which glial Ca-permeable AMPA receptors are turned Ca-impermeable during cerebellar development. Using lentiviral vectors with truncated GFAP promoters, I succeeded in expressing a GluR2 gene in glial cells effectively, with no change in tropism.