Development of MR microscope for organogenesis research

开发用于器官发生研究的 MR 显微镜

• 批准号: 22700470
• 负责人: UENO Tomohiro
• 金额: $ 2.5万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22700470/
• 关键词: MRI(磁気共鳴映像法); 顕微鏡; 磁気共鳴映像法; MRI

We developed a magnetic resonance microscope by using high magnetic field of 14.1 T. We visualized chemically-fixed medaka in 20 μm isovoxel three dimensionally, where field gradients of 4.0 T/m at maximum were applied. Comparing 3D MRimages of p53 cancer suppressor gene mutant medaka with those of wild type medaka, differences in internal organs were obtained. In addition to that, we analyzed digitized effects in quantitative susceptibility mapping, which gave us a tissue iron contentmap and could be used for detecting local iron accumulation in a brain of a Parkinson’s disease patient.

我们使用 14.1 T 的高磁场开发了磁共振显微镜。我们在 20 μm 等像素中对化学固定的青鳉进行三维可视化，其中应用的场梯度最大为 4.0 T/m。通过比较 p53 抑癌基因突变青鳉与野生型青鳉的 3D MR 图像，获得了内脏器官的差异。除此之外，我们还分析了定量磁化率绘图中的数字化效应，这为我们提供了组织铁含量图，可用于检测帕金森病患者大脑中的局部铁积累。

项目成果

Effective Digitized Spatial Size of Unit Dipole Field in Quantitative Susceptibility Mapping

定量磁化率绘图中单位偶极子场的有效数字化空间尺寸

• 发表时间: 2013
• 期刊: the proceedings of 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society
• 作者: Mai Murashima;Tomohiro Ueno;and Naozo Sugimoto
• 通讯作者: and Naozo Sugimoto

MRI顕微鏡によるメダカガン細胞のin situ 3次元可視化に向けた固定法の検討

MRI显微镜下青鳉癌细胞原位3D可视化固定方法研究

• 发表时间: 2010
• 作者: Takeshita D;Nakajima-Takenaka C;Shimizu J;Hattori H;Nakashima T;Kikuta A;Matsuyoshi H;Takaki M.;桑原航
• 通讯作者: 桑原航

Postmortem 3D Image Comparison of Wild Type and p53 Knockout Medaka by MR Microscope

通过 MR 显微镜对野生型和 p53 基因敲除青鳉进行尸检 3D 图像比较

• 发表时间: 2012
• 期刊: Molecular Imaging and Biology
• 影响因子: 3.1
• 作者: M.Murashima;T. Ueno;K. Sakai;Y. Taniguchi;and N. Sugimoto
• 通讯作者: and N. Sugimoto

3D Visualization of the Medaka by MR Microscope

通过 MR 显微镜对青鳉进行 3D 可视化

• 发表时间: 2010
• 作者: T.Kawashima;T.Shinmachi;T.Shibata;M.Nomura;T.Kimura;A.Kishida;T.Mineta;E.Makino;T.Ueno
• 通讯作者: T.Ueno

Effective Digitized Spatial Size of Unit Dipole Field in Quantitative Susceptibility Mapping

定量磁化率绘图中单位偶极子场的有效数字化空间尺寸

• 发表时间: 2013
• 期刊: the proceedings of 35th Annual International Conference of the IEEE
• 作者: Mai Murashima;Tomohiro Ueno;and Naozo Sugimoto
• 通讯作者: and Naozo Sugimoto