Oxidative stress signal function in stem cells via keap1-Nrf2 system

通过keap1-Nrf2系统在干细胞中发挥氧化应激信号作用

• 批准号: 22700870
• 负责人: MORITA Masanobu
• 金额: $ 2.58万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22700870/
• 关键词: がん微小環境

New ES cells lines are generated from Nrf2, Keap1 null blastocytes. These Nrf2 KO and Keap1 KO ES cells are expected to have phenotypes in oxidative stress responses. Nrf2 and Keap1 null blastocytes are obtained from cross mating heterozygous mutant mice. Genomic PCR are used to determine the genotypes of established ES cells. Nrf2 and Keap1 null ES cells show typical ES-like morphologies. The ES cell lines also show expression pattern of ES cell markers. Embryoid body formation are used as ES differentiation system.

新的ES细胞系由Nrf2、Keap 1无效胚细胞产生。这些Nrf2 KO和Keap1 KO ES细胞预期在氧化应激反应中具有表型。Nrf2和Keap 1无效胚细胞获自杂交杂合突变小鼠。基因组PCR用于确定建立的ES细胞的基因型。Nrf2和Keap1缺失的ES细胞表现出典型的ES样形态。ES细胞系也显示ES细胞标志物的表达模式。胚状体的形成被用作ES分化系统。

项目成果

Survival Strategy and Disease Pathogenesis According to the Nrf2‐Small Maf Heterodimer

根据 Nrf2-Small Maf 异二聚体的生存策略和疾病发病机制

• DOI: 10.1002/9781118148143.ch5
• 发表时间: 2011
• 期刊: Nanoscale
• 影响因子: 6.7
• 作者: Masanobu Morita;H. Motohashi
• 通讯作者: H. Motohashi