Oxidative stress signal function in stem cells via keap1-Nrf2 system

新的ES细胞系由Nrf2、Keap 1无效胚细胞产生。这些Nrf2 KO和Keap1 KO ES细胞预期在氧化应激反应中具有表型。Nrf2和Keap 1无效胚细胞获自杂交杂合突变小鼠。基因组PCR用于确定建立的ES细胞的基因型。Nrf 2和Keap 1缺失的ES细胞显示出典型的ES样形态。ES细胞系也显示ES细胞标志物的表达模式。胚状体的形成被用作ES分化系统。

New ES cells lines are generated from Nrf2, Keap1 null blastocytes. These Nrf2 KO and Keap1 KO ES cells are expected to have phenotypes in oxidative stress responses. Nrf2 and Keap1 null blastocytes are obtained from cross mating heterozygous mutant mice. Genomic PCR are used to determine the genotypes of established ES cells. Nrf2 and Keap1 null ES cells show typical ES-like morphologies. The ES cell lines also show expression pattern of ES cell markers. Embryoid body formation are used as ES differentiation system.