The investigation of function of repressor Bach1 in microenvironment of the tumor and the application to a mouse model for cancer stem cell transplantation.

我们计划构建 Bach1^</-/-> 免疫缺陷小鼠作为人类可移植肿瘤细胞植入和转移的良好受体小鼠模型。因此，我们旨在研究Bach1在成纤维细胞中的机制和作用。为了鉴定成纤维细胞中 Bach1 的新靶基因，我们进行了 ChIP-seq、DNA 微阵列、qPCR 和蛋白质印迹分析。这些结果表明，Bach1 在永生化 MEF (iMEF) 中充当 Pparγ 基因转录的抑制子。在体外用胰岛素、IBMX、地塞米松和 Pparγ 配体处理时，Bach1^</-/-> iMEF 更容易分化为脂肪细胞。 Bach1可能通过调节Pparγ的作用在成纤维细胞的脂肪分化中发挥作用。

We planned the construction of a Bach1^<-/-> immune-deficient mouse as a good recipient mouse model for engraftment and metastatic of human transplantable tumor cells. Therefore, we aimed to investigate the mechanism and role of Bach1 in fibroblast cell. To identify novel target genes of Bach1 in fibroblast cell, we performed ChIP-seq, DNA microarray, qPCR and western blotting analysis. These results suggest that Bach1 acts as a repressor of Pparγgene transcription in immortalized MEF (iMEF). Bach1^<-/-> iMEF more easily differentiated to adipocytes when treated with insuline, IBMX, dexamethasone and Pparγ ligand in vitro. Bach1 might play a role in adipose differentiation of fibroblast cell by modulating the actions of Pparγ.