The investigation of function of repressor Bach1 in microenvironment of the tumor and the application to a mouse model for cancer stem cell transplantation.

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We planned the construction of a Bach1^<-/-> immune-deficient mouse as a good recipient mouse model for engraftment and metastatic of human transplantable tumor cells. Therefore, we aimed to investigate the mechanism and role of Bach1 in fibroblast cell. To identify novel target genes of Bach1 in fibroblast cell, we performed ChIP-seq, DNA microarray, qPCR and western blotting analysis. These results suggest that Bach1 acts as a repressor of Pparγgene transcription in immortalized MEF (iMEF). Bach1^<-/-> iMEF more easily differentiated to adipocytes when treated with insuline, IBMX, dexamethasone and Pparγ ligand in vitro. Bach1 might play a role in adipose differentiation of fibroblast cell by modulating the actions of Pparγ.