Two-photon imaging analysis of cytotoxic T cell-mediated anti-tumor immune responses

细胞毒性 T 细胞介导的抗肿瘤免疫反应的双光子成像分析

• 批准号: 22700901
• 负责人: KITANO Masahiro
• 金额: $ 2.5万
• 依托单位: The Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22700901/
• 关键词: 二光子; イメージング; 癌免疫

We have established the intravital multi-photon imaging system for monitoring the cell behavior of antigen-specific cytotoxic T cells and endogenous dendritic cells in living mouse lymph node and tumor. By using this system, we have visualized the entire process of T cell activation through interactions with endogenous dendritic cells, and identified a dendritic cell subset responsible for antigen presentation selectively to cytotoxic T cells. Furthermore, we have found antigen-specific interactions between tumor-infiltrating effector-cytotoxic T cells and tumor-surrounding dendritic cells, suggesting a new mechanism for inducing T cell proliferation and differentiation not only at the secondary lymphoid organs, but also at the effector sites such as tumor lesion.

我们建立了活体多光子成像系统，用于监测活体小鼠淋巴结和肿瘤中抗原特异性细胞毒性T细胞和内源性树突状细胞的细胞行为。通过使用该系统，我们通过与内源性树突状细胞相互作用观察了 T 细胞激活的整个过程，并确定了负责选择性向细胞毒性 T 细胞呈递抗原的树突状细胞亚群。此外，我们发现肿瘤浸润效应细胞毒性T细胞和肿瘤周围树突状细胞之间存在抗原特异性相互作用，这表明不仅在次级淋巴器官，而且在肿瘤病变等效应部位诱导T细胞增殖和分化的新机制。

项目成果

FOUR-DIMENSIONAL TRACKING OF LYMPHOCYTE MIGRATION AND INTERACTIONS BY TWO-PHOTON MICROSCOPY

通过二维光子显微镜对淋巴细胞迁移和相互作用进行四维追踪

• DOI: 10.1016/b978-0-12-391856-7.00047-0
• 发表时间: 2012
• 期刊: Methods in Enzymology
• 作者: Kitano;M.;Okada;T.
• 通讯作者: T.

Four-dimensional tracking of lymphocyte migration and interactions in lymph nodes by two-photon microscopy

双光子显微镜对淋巴结中淋巴细胞迁移和相互作用的四维追踪

• 发表时间: 2012
• 期刊: Methods in Enzymology
• 作者: Kitano;M. and Okada;T
• 通讯作者: T

BCL6 Expression Dynamics in the Lymph Node during the Antibody Response

抗体反应期间淋巴结中 BCL6 表达动态

• 发表时间: 2010
• 作者: Kitano;M
• 通讯作者: M