Establishment of Glioma stem-like cells(GSC) from glioma patient tumors and analysis of the novel molecular markers of GSC by an integrated proteomics.

胶质瘤干细胞（GSC）被认为是恶性胶质瘤治疗耐药和复发的原因。调节这些细胞的维持/分化的分子被认为是有效的治疗靶标。在这项研究中，我们建立了恶性胶质瘤患者的肿瘤GSC克隆，并对其进行整合蛋白质组学调查GSC差异表达的蛋白质和mRNA后，他们已被血清刺激。这导致在30000分子的鉴定和GO分析表明，细胞粘附分子，包括整合素亚家族成员和细胞外基质（ECM）的表达显着上调GSC分化过程中。经过细胞生物学验证，我们发现血清因子诱导的ECM与分化和增殖偶联。这些结果提示GSC可能通过自身诱导分泌ECM，形成一个特殊的微环境，称为分化小生境，从而促进恶性胶质瘤的发生。

Glioma stem cells(GSCs) are thought to be responsible for the therapeutic resistance and recurrence of malignant glioma. The molecules regulating the maintenance/differentiation of these cells are considered effective therapeutic targets. In this study, we established GSC clones from tumors of malignant glioma patients and subjected them to integrated proteomics to survey the proteins and mRNAs differentially expressed in GSCs after they have been induced by serum stimulation. This resulted in the identification of 30000 molecules and GO analysis revealed that the expression of cell adhesion molecules, including integrin subfamily members and extracellular matrices(ECMs) was significantly upregulated during GSC differentiation. After the cell-biological validations, we found that serum factor-induced coupling of ECMs to differentiation and proliferation. The results raised the possibility that GSC induces/secretes ECMs by itself to form a specific microenvironment, called the "differention niche", which facilitates the development of malignant glioma.