Establishment of Glioma stem-like cells(GSC) from glioma patient tumors and analysis of the novel molecular markers of GSC by an integrated proteomics.

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Glioma stem cells(GSCs) are thought to be responsible for the therapeutic resistance and recurrence of malignant glioma. The molecules regulating the maintenance/differentiation of these cells are considered effective therapeutic targets. In this study, we established GSC clones from tumors of malignant glioma patients and subjected them to integrated proteomics to survey the proteins and mRNAs differentially expressed in GSCs after they have been induced by serum stimulation. This resulted in the identification of 30000 molecules and GO analysis revealed that the expression of cell adhesion molecules, including integrin subfamily members and extracellular matrices(ECMs) was significantly upregulated during GSC differentiation. After the cell-biological validations, we found that serum factor-induced coupling of ECMs to differentiation and proliferation. The results raised the possibility that GSC induces/secretes ECMs by itself to form a specific microenvironment, called the "differention niche", which facilitates the development of malignant glioma.