Visualization of spatiotemporal metabolic dynamics in tissue micro-region

我们成功地显示了基质辅助激光解吸电离质谱（MALDI-MS）系统的适用性，用于在单个哺乳动物细胞上获得化学上不同的代谢产物谱。这种超高灵敏度的MALDI-MS技术能够对广泛的代谢物进行空间分辨检测，包括核苷酸、辅因子、磷酸化糖、氨基酸、脂质和具有独特分布的羧酸。MS成像和代谢途径分析的组合可视化的代谢产物的时空行为的中枢代谢途径调节缺血再灌注大鼠脑。此外，我们开发了一种新的策略，用于确定有机化合物的元素组成使用的同位素精细结构的峰比观察到的100质量分辨率质谱数据。

We successfully showed the applicability of a matrix-assisted laser desorption ionization mass spectrometry(MALDI-MS) system for getting chemically diverse metabolite profiles on a single-mammalian cell. This ultrahighly sensitive MALDI-MS technique enabled a spatially resolved detection of a broad range of metabolites including nucleotides, cofactors, phosphorylated sugars, amino acids, lipids, and carboxylic acids with their unique distributions. A combination of MS imaging and metabolic pathway analysis visualized a spatiotemporal behavior of metabolites in the central metabolic pathway regulated by an ischemia reperfusion in rat brain. Furthermore, we developed a novel strategy for determining the elemental composition of organic compounds using the peak ratio of isotopic fine structure observed by ultrahigh mass resolution mass spectrometric data.