Metabolite profiling in rice plant by Multidimensional NMR-Based Metabolomics
Metabolite profiling in rice plant by Multidimensional NMR-Based Metabolomics
批准号:
22710230
负责人:
SEKIYAMA Yasuyo
金额:
$2.25万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Young Scientists (B)
财政年份:
2010
资助国家:
日本
项目状态:
已结题
起止时间:
2010 至 2011
中文摘要
^<13> C标记允许增加代谢物注释的可靠性。为了扩大水稻代谢物谱的信息,我尝试通过^<13>的CO_2饲喂产生高^<13> c标记的水稻植株。通过1^H核磁共振光谱中蔗糖的球端质子信号估计,被标记植物的标记率为75%。从标准数据库中列出候选极性和半极性代谢物。
英文摘要
^<13> C labeling allows to increase the reliability of metabolite annotation. In an attempt to expand information on metabolite profiles in Oryza sativa, I tried to generate highly^<13> C-labeled rice plants by^<13> CO_2 feeding. The labeling ratio of the labeled plant estimated from the anomeric proton signal of sucrose in the 1^H NMR spectra was 75%. Candidate polar and semipolar metabolites in the leaves were listed from the standard database.
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专著(0)
科研奖励(0)
会议论文
Evaluation of a Semipolar Solvent System as a Step toward Heteronuclear Multidimensional NMR-Based Metabolomics for C^<13>-Labeled Bacteria
评估半极性溶剂系统作为 C^13 标记细菌异核多维 NMR 代谢组学的一个步骤
DOI:
--
发表时间:
2011
期刊:
Plants, and Animals, Analytical Chemistry
影响因子:
--
作者:
[Yasuyo Sekiyama, Eisuke Chikayama, Jun Kikuchi]
通讯作者:
Jun Kikuchi
NMR法によるメタボローム解析の現状と農作物解析への応用
NMR方法代谢组分析的现状及其在作物分析中的应用
DOI:
--
发表时间:
2012
期刊:
影响因子:
--
作者:
[Kusavanagi T, Tsukuda S, Shimura S, Manita D, Iwakiri K, Kamisuki S, Takakusagi Y, Takeuchi T, Kuramochi K, Nakazaki A, Sakaguchi K, Kobayashi S, Sugawara F, 関山恭代]
通讯作者:
関山恭代
Application of NMR-based metabolomics to the development of marker-assisted breeding and new cultivation technique
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批准号:15K14643
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项目类别:Grant-in-Aid for Challenging Exploratory Research
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资助金额:$2.5万
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财政年份:2015
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负责人:SEKIYAMA Yasuyo
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依托单位:
海外基金