Diversity of mycolic acid containing actinomycetes isolated from subtropical region in Japan and its conservation

本研究旨在揭示日本亚热带地区含放线菌如诺卡氏菌和分枝杆菌的多样性和保存性。森林和农田土壤样品采集于日本冲绳的伊洛莫特岛。采用蔗糖梯度离心法和油-十二烷基硫酸钠乳化法对诺卡氏菌和分枝杆菌进行选择性分离。检出诺卡氏菌12种，分枝杆菌2种。红球菌属的选择性分离方法是联合使用抗生素(发酵霉素20 mg/L和新诺菌素10 mg/L)和用碱性十二烷基硫酸钠溶液对土壤进行预处理。其中一些菌株被怀疑为新种，并被保存在NITE-生物资源中心微生物保护中心

The aim of this study was to reveal the diversity and conservation of mycolic acid containing actinomycete such as Nocardia and Mycobacterium from subtropical area in Japan. Forest and cultivated field soil samples were collected from Iriomote island, Okinawa, Japan. The isolates belonging to the genus Nocardia and Mycobacterium were selectively isolated using sucrose-gradient centrifugation method and Oil-SDS emulsification method. Twelve species of Nocardia and two species of Mycobacterium was detected. Selective isolation method for the genus Rhodococcus was developed as combined use of antibiotics(fradiomycine ; 20 mg/L and novobiocin ; 10 mg/L) and pretreatment of soil with alkaline-SDS solution. Some of isolates were suspected as new species and were deposited in NITE-Biological Resource Center for microbial conservation