Diversity of mycolic acid containing actinomycetes isolated from subtropical region in Japan and its conservation

本研究旨在揭示日本亚热带地区含分枝菌酸放线菌如诺卡氏菌和分枝杆菌的多样性和保守性。从日本冲绳西表岛采集森林和耕地土壤样品。采用蔗糖梯度离心法和油-SDS乳化法对诺卡氏菌和分枝杆菌进行了分离。检出诺卡氏菌12种，分枝杆菌2种。采用抗生素（20 mg/L的新霉素和10 mg/L的新生霉素）联合应用和碱性SDS溶液预处理的方法，对红球菌属进行了分离。部分菌株被怀疑为新种，并保存在NITE-Biological Resource Center进行微生物资源保护

The aim of this study was to reveal the diversity and conservation of mycolic acid containing actinomycete such as Nocardia and Mycobacterium from subtropical area in Japan. Forest and cultivated field soil samples were collected from Iriomote island, Okinawa, Japan. The isolates belonging to the genus Nocardia and Mycobacterium were selectively isolated using sucrose-gradient centrifugation method and Oil-SDS emulsification method. Twelve species of Nocardia and two species of Mycobacterium was detected. Selective isolation method for the genus Rhodococcus was developed as combined use of antibiotics(fradiomycine ; 20 mg/L and novobiocin ; 10 mg/L) and pretreatment of soil with alkaline-SDS solution. Some of isolates were suspected as new species and were deposited in NITE-Biological Resource Center for microbial conservation