Mitochondrial targeting signal is major determinant of mitochondrial localizing mRNA

线粒体靶向信号是线粒体定位 mRNA 的主要决定因素

• 批准号: 23300112
• 负责人: HORTON Paul
• 金额: $ 12.81万
• 依托单位: National Institute of Advanced Industrial Science and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23300112/
• 关键词: mRNA局在; ミトコンドリア; mRNA配列解析; タンパク質局在

Mitochondria dysfunction has been implicated in Alzheimer's and other diseases, but the cellular mechanisms used to maintain mitochondria are only partially understood. Thus we investigated the transport of proteins into the mitochondria and quantitatively showed that a particular region of those proteins evolves faster than other proteins (Fukasawa et al. BMC Genomics 2014). In addition we investigated the localization of mRNA molecules in the vicinity of mitochondria. We did this by integrative statistical analysis of several published datasets. Based on the results of our analysis we propose a novel hypothesis -- that the longer it takes for proteins to be made (translation initiation), the more likely it is that they localize to the periphery of the mitochondria. By providing insights into the mechanisms responsible for maintenance of mitochondrial, in the future our results may contribute to advances in the prevention or treatment of mitochondria related disease.

线粒体功能障碍与阿尔茨海默氏症和其他疾病有关，但用于维持线粒体的细胞机制只被部分了解。因此，我们研究了蛋白质向线粒体的运输，并定量地表明，这些蛋白质的特定区域比其他蛋白质进化得更快(Fukasawa等人)。BMC基因组学2014)。此外，我们还研究了mRNA分子在线粒体附近的定位。我们通过对几个已发表的数据集进行综合统计分析来做到这一点。基于我们的分析结果，我们提出了一个新的假设--蛋白质合成(翻译启动)的时间越长，它们就越有可能定位在线粒体的外围。通过提供对线粒体维持机制的见解，我们的结果可能在未来有助于在预防或治疗线粒体相关疾病方面取得进展。

项目成果

Advances in Predictiing Protein Sub-cellular Localization Signals

预测蛋白质亚细胞定位信号的进展

• 发表时间: 2013
• 作者: 水田淳史;葛 崎偉;松野浩嗣;Paul Horton
• 通讯作者: Paul Horton

Utilizing probabilistic dependencies in low-complexity regions when detecting homologous regions of biological sequences.

在检测生物序列的同源区域时利用低复杂性区域中的概率依赖性。

• 发表时间: 2012
• 作者: 岩木 直;原田 暢善;松本唯尊;樋口 知之;Poulsen Thomas
• 通讯作者: Poulsen Thomas

Recent Advances in Understanding Protein Sub-cellular Localization Signals

了解蛋白质亚细胞定位信号的最新进展

• 发表时间: 2013
• 作者: Hiroshi Matsuno;Makoto Akashi;S. Iwaki;Paul Horton
• 通讯作者: Paul Horton

Mitochondrial targeting signal is major determinant of mitochondrial localizing mRNA

线粒体靶向信号是线粒体定位 mRNA 的主要决定因素

• 发表时间: 2011
• 作者: 崎山 則征;今井 賢一郎;富井 健太郎
• 通讯作者: 富井 健太郎

Plus ça change - evolutionary sequence divergence predicts protein subcellular localization signals.

• DOI: 10.1186/1471-2164-15-46
• 发表时间: 2014-01-20
• 期刊: BMC genomics
• 影响因子: 4.4
• 作者: Fukasawa Y;Leung RK;Tsui SK;Horton P
• 通讯作者: Horton P