Development of single chain antibodies against sugar chain tumor antigen on the surface of leukemia cells and their application for order-made medicine

白血病细胞表面糖链肿瘤抗原单链抗体的研制及其在定制药物中的应用

• 批准号: 23300356
• 负责人: SUDA Yasuo
• 金额: $ 14.39万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23300356/
• 关键词: 腫瘍マーカー; オーダーメード治療

Specifically expressed sugar chains on adult T-cell leukemia(ATL) cells should be tumor markers,which can be appropriate antigen molecules in the targeted therapy using ant ibodies.Trace amount of sugar chains extracted and fractionated from tumor ATL cells were immobilized on the top of the optical fiber(50) chemically modified with gold nanoparticles to prepare fiber-type sugar chip. Using the chip and localized surface Plasmon resonance method,the screening of phage display was performed to obtain phages expressing single chain antibody(scFV)which bound to sugar chain from ATL cells. then,severel scFv were isolated from the selected phages.One scFv was then immobilized to fluorescent nanoparticles(FNP) with a optimal conditions to prepare scFv-FNP. By the FACS analysis and fluorescent microscopy,the scFv-FNP was found to bind specifically to cultured ATL cell(S1T),but not to non ATL leukemia cells(CEM),indicating the easy detection tool for ATL cells.

成人T细胞白血病（ATL）细胞上特异表达的糖链应是肿瘤标志物，可作为抗肿瘤抗体靶向治疗中的合适抗原分子，将从肿瘤ATL细胞中提取并分级分离的微量糖链固定在用纳米金化学修饰的光纤（50）的顶部，制备纤维型糖芯片。利用该芯片和定位表面等离子体共振技术，从ATL细胞中筛选出能与糖链结合的单链抗体（scFV）。然后，从筛选出的噬菌体中分离出噬菌体单链抗体，并将其固定于荧光纳米颗粒（FNP）上，制备出scFv-FNP。通过流式细胞仪和荧光显微镜观察，发现scFv-FNP与培养的ATL细胞（S1 T）特异性结合，而与非ATL白血病细胞（CEM）不结合，表明scFv-FNP是ATL细胞的简便检测工具。

项目成果

ビフィズス菌由来β-L-アラビノビオシダーゼの機能解析

双歧杆菌β-L-阿拉伯二糖苷酶的功能分析

• 发表时间: 2011
• 期刊: 応用糖質科学
• 作者: 藤田清貴;坂元志帆;小野祐樹;若尾雅広;隅田泰生;北原兼文;菅沼俊彦
• 通讯作者: 菅沼俊彦

Vincristine exacerbates asymptomatic Charcot-Marie-Tooth disease with a novel EGR2 mutation

• DOI: 10.1007/s10048-012-0313-1
• 发表时间: 2012-02-01
• 期刊: NEUROGENETICS
• 影响因子: 2.2
• 作者: Nakamura, Tomonori;Hashiguchi, Akihiro;Takashima, Hiroshi
• 通讯作者: Takashima, Hiroshi

Effects of exogenous interleukin-7 on CD8(+) T-cell survival and function in human T-cell lymphotropic virus type 1 infection.

外源性白细胞介素 7 对人 T 细胞嗜淋巴细胞病毒 1 型感染中 CD8(+) T 细胞存活和功能的影响。

• DOI: 10.3109/10428194.2013.772174
• 发表时间: 2013
• 期刊: Leukemia and Lymphoma.
• 作者: White Y;Yoshimitsu M;Kozako T;Matsushita K;Koriyama C;Uozumi K; Suzuki S;Kofune H;Arima N.
• 通讯作者: Arima N.

T7ファージペプチドライブラリを用いたヒトIgG結合性ペプチドの単離と親和性増強

使用 T7 噬菌体肽库分离和增强人 IgG 结合肽

• 作者: 中島田雄一;畠中孝彰;北尾みどり;大薗慎二;伊東治;空處弘一;有馬一成;伊東祐二
• 通讯作者: 伊東祐二

ファージディスプレイによるブタ抗体ライブラリの構築と評価

噬菌体展示猪抗体库的构建与评价

• 作者: 若井純子;小澤拓矢;野元花愛;有馬一成，伊東祐二
• 通讯作者: 有馬一成，伊東祐二