ADF-STEM Analysis of Magnetic Particles in Bio-system without Staining

生物系统中磁性粒子的 ADF-STEM 分析（无需染色）

• 批准号: 23310075
• 负责人: ISODA Seiji
• 金额: $ 2万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-11-18 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23310075/
• 关键词: 無染色可視化; 暗視野電子顕微鏡; ソフトマテリアル; メゾスコピック系; 可視化; 走査型透過電子顕微鏡; メゾスコピック; 磁性粒子; 細胞・組織

In the transfer mechanism of external particles into the biological system, the identification of pathways and final accumulation sites is essential for the understanding of the interaction of particles and biological systems. The aim of this study was to propose a methodology for high-resolution electron microscopic analysis of biological samples for realizing the direct spatial distribution analysis of the particle transfer.In this study, a scanning transmission electron microscope dark field method was proposed for the analysis of a specimen mainly composed of light elements such as biological cells, by which we proposed a non-staining and also thick sample analysis. As a result, it was possible to analyze the localization of microparticles in cell samples introduced with magnetic particles by means of the non-staining electron microscopy,.

在外部粒子进入生物系统的转移机制中，识别路径和最终积累位点对于理解粒子与生物系统的相互作用至关重要。本研究的目的是提出一种用于生物样品的高分辨电子显微镜分析的方法，以实现直接的粒子转移的空间分布分析，在本研究中，提出了一种扫描透射电子显微镜暗场方法，用于分析主要由轻元素组成的样品，如生物细胞，我们提出了一种非染色和厚样品分析。结果，可以通过非染色电子显微镜分析引入磁性颗粒的细胞样品中微粒的定位。

项目成果

Quantitative Imaging of Micro-phases in Copolymers by LAADF-STEM

通过 LAADF-STEM 对共聚物中的微相进行定量成像

• 发表时间: 2013
• 作者: Ryotaro Aso;Hiroki Kurata;and Seiji Isoda
• 通讯作者: and Seiji Isoda

Molecular imaging by electron microscopy

电子显微镜分子成像

• 发表时间: 2013
• 作者: K. Kawaguchi;H. Ikeda;D. Sakai;K. Harada;S. Akiba;T. Suzuki and J. Nishii;S. Isoda and H.Kurata
• 通讯作者: S. Isoda and H.Kurata

Quantitative Imaging of Tg in Block Copolymers by Low-Angle Annular Dark-Field Scanning Transmission Electron Microscopy

• DOI: 10.1021/ma4014934
• 发表时间: 2013-10
• 期刊: Macromolecules
• 影响因子: 5.5
• 作者: R. Aso;H. Kurata;Takeshi Namikoshi;T. Hashimoto;S. Kuo;F. Chang;H. Hasegawa;M. Tsujimoto;M. Takano;S. Isoda

Atomic resolution imaging of organic crystals by STEM

利用 STEM 对有机晶体进行原子分辨率成像

• 发表时间: 2012
• 作者: Mitsutaka Haruta;Seiji Isoda and Hiroki Kurata
• 通讯作者: Seiji Isoda and Hiroki Kurata

ADF-STEM Analysis for Soft Materials

软材料的 ADF-STEM 分析

• 发表时间: 2012
• 作者: Ryotaro Aso;Masahiko Tsujimoto;Hiroki Kurata;and Seiji Isoda
• 通讯作者: and Seiji Isoda