Analysis of prophyrin-mediated signaling pathways for the control of nuclear genes by the chloroplast

叶绿体控制核基因的卟啉介导的信号通路分析

• 批准号: 5402423
• 负责人: Professor Dr. Christoph Friedrich Beck
• 金额: --
• 依托单位: Institut für Biologie III
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2003
• 资助国家: 德国
• 起止时间: 2002-12-31 至 2008-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5402423?language=en
• 关键词: Analysis; prophyrin; mediated; signaling; pathways

The project is focussed on one aspect of intracellular communication: the signaling from chloroplast to nucleus. Studies with higher plants and our work with Chlamydomonas have provided evidence for a role of tetrapyrroles and of enzymes involved in their biosynthesis in chloroplast-to-nucleus signaling. The goal of the project is the molecular and functional characterization of genes and molecules that mediate this communication. For this purpose, we will identify mutants defective in this communication and characterize the altered genes and their products. In a complementing approach, an enhancer sequence that responds to a chloroplast signal will be employed to characterize protein(s) that bind to it. And in mutants that exhibit a deregulation in both, their chlorophyll precursor levels and in the expression of nuclear genes involved in tetrapyrrole biosynthesis, we plan to dissect the basis for the altered signaling from chloroplast to nucleus.

该项目的重点是细胞内通讯的一个方面：从叶绿体到细胞核的信号。对高等植物的研究和我们对衣原体的研究提供了四吡咯和参与其生物合成的酶在叶绿体到细胞核信号传导中的作用的证据。该项目的目标是介导这种通信的基因和分子的分子和功能表征。为此，我们将确定在这种通信缺陷的突变体，并表征改变的基因及其产物。在一个互补的方法，增强子序列，响应叶绿体信号将被用来表征蛋白质（S）的绑定到it.And在突变体中表现出失调，在这两个，他们的叶绿素前体水平和参与四吡咯生物合成的核基因的表达，我们计划解剖的基础上改变信号从叶绿体到核。