Development of high throughput image cytometry for microalgal screening toward oil production

建立了高通量图像细胞术筛选高产微藻的方法。微腔培养装置形成大尺度的细胞图谱，然后延时成像观察细胞生长和油脂积累情况。此外，还开发了采用CMOS图像传感器的蜂群形成监测系统。它提高了观察通量，使我们能够克服目前微藻筛选的局限性。本研究开发的图像细胞仪系统有望用于探索有前途的生物燃料微藻生产者，以及其他可变材料，包括类胡萝卜素，鞘糖脂和多不饱和脂。

High throughput image cytometry was developed for screening of microalgae with high oil production. The microcavity-culture device created large scale cell patterning, and then time-lapse imaging was performed to observe cell growth and oil accumulation. Additionally, colony formation monitoring system was also developed using a CMOS image sensor. It improved observation throughput, which allowed us to overcome current limitation of the microalgal screening. The image cytometry system developed in this study is expected to be used to explore promising microalgal producers of biofuel, as well as other variable materials including carotenoid, glycosphingolipid and polyunsaturated lipid.