Detection, identification and mechanism of defense-related molecule for plant parasitic nematodes.

我们将研究重点放在紫草醇上，这是一种天然的二萜类化合物，被称为抗菌剂和防御相关分子，并研究了在植物体内应用紫草醇是否以及如何抑制RKN对番茄和拟南芥根的侵染。除了乙烯(ET)不敏感突变体ein2-1外，接受sclareol处理的植株抑制了RKN的侵染。外源sclareol增强了EIN2依赖的途径，并诱导了木质素生物合成基因在植物根中的表达。苯丙烷途径的激活导致木质素在根中积累，从而抑制了RKN的入侵。在木质素生物合成基因缺失的拟南芥突变体中，Sclareol诱导的RKN抗性被削弱，这表明木质素参与了Sclareol诱导的对RKN的抗性。

We focused on sclareol, a natural labdane-type diterpene known as the antimicrobial and defense-related molecule, and investigated whether and how sclareol application on plant body inhibits RKN infection to tomato and Arabidopsis roots. Plants that received sclareol application suppressed RKN infection, except for the ethylene (ET) insensitive mutant ein2-1.Exogenously applied sclareol enhanced EIN2-dependent pathway and induced expression of lignin biosynthetic genes in roots of the plant. Accumulation of lignin in the roots which was caused by the activation of phenylpropanoid pathway resulted in suppression of invasion of RKN. Sclareol-induced RKN resistance was compromised in the Arabidopsis mutant deficient in a lignin biosynthetic gene, suggesting that lignin is involved in the sclareol-induced resistance against RKN.