Monitoring of heartwood formation by histochemistry and tree-stem vibration property

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The objectives of this study was a technical development for the monitoring of the process of heartwood formation in trees by means of histochemistry and vibrational property of tree trunk. Two species, Cryptomeria japonica and Larix kaempferi, were investigated because of their importance in Japanese forestry. The new and improved methods and new findings have been achieved for the observation and measurement of heartwood formation in minute details: morphological and histochemical change of nuclei in ray parenchyma during heartwood formation; in situ localization of major heartwood substances in L. kaempferi; estimation of the amount of water in tree trunk measured by mechanical vibration and electrical impedance; changes of cell wall with fine histochemical anatomy.