Elucidation of mechanism of neuroendocrine control in reproduction for advancement of artificial seed production of commercial bivalve mollusks

阐明繁殖中神经内分泌控制机制，促进商业双壳类软体动物人工种子生产

• 批准号: 23380109
• 负责人: MAKOTO Osada
• 金额: $ 12.15万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23380109/
• 关键词: 二枚貝; 神経内分泌; エストロゲンシグナリング; 配偶子形成; 産卵; エストロゲンシグナリン

We cloned the cDNA transcripts of prepro Gonadotropin Releasing Hormones (GnRH)-like peptides from the central nervous system of scallop(pyGnRH)and oyster (cgGnRH). The putative pyGnRH peptide stimulated the proliferation of spermatogonia, which was mediated through the induction of estrogen synthesis by pyGnRH.We successfully cloned two novel forms of estrogen receptor from two kinds of mussels and Japanese scallop. These receptors were classified each into an estrogen receptor (ER) and estrogen-related receptor (ERR) and were expressed in both ovary and testis. The flounder ERa-mediated gene assay suggested that pyER behaves as a transcription factor to estrogen while pyERR is a constitutive activator.Serotonin (5-HT)-induced oocyte maturation and sperm motility is inhibited by oocyte maturation arresting factor (OMAF) due to interference of extracellular Ca2+ influx. The release from suppressive OMAF due to absorption with antibody against OMAF amplified the 5-HT-induced spawning.

我们克隆了扇贝(PyGnRH)和牡蛎(CgGnRH)中枢神经系统促性腺激素释放激素前体(GnRH)样多肽的cDNA转录产物。我们成功地从两种贻贝和日本扇贝中克隆了两种新的雌激素受体。这些受体分为雌激素受体(ER)和雌激素相关受体(ERR)，在卵巢和睾丸中均有表达。比目鱼Era介导的基因分析表明，Pyer是雌激素的转录因子，而Pyer是结构性激活剂。5-羟色胺(5-HT)诱导的卵母细胞成熟和精子运动被卵母细胞成熟抑制因子(OMAF)抑制，这是由于细胞外钙内流的干扰。抑制的OMAF由于被抗体吸收而释放，放大了5-羟色胺诱导的产卵。

项目成果

Bivalve Mollusc Gonadotropin-Releasing Hormone

双壳类软体动物促性腺激素释放激素

• 发表时间: 2011
• 作者: Nicholas Treen;Naoki Itoh;Hanae Miura;Ippei Kikuchi;Takenori Ueda;Keisuke G.Takahashi ;Takayoshi Ubuka ;Kazutoshi Yamamoto ;Kazuyoshi Tsutsui ;Peter Sharp ;Makoto Osada
• 通讯作者: Makoto Osada

海産二枚貝エストロゲン受容体の機能解析

海洋双壳类雌激素受体的功能分析

• 发表时间: 2012
• 作者: 乙木百合香;Emily Marquez;伊藤直樹;高橋計介;尾定誠
• 通讯作者: 尾定誠

Primary structural analyais of estrogen receptors in marine bivalves, The Seventh Congress of the Asia and Oceania Society for Comparative Endocrinology (AOSCE)

海洋双壳类雌激素受体的初级结构分析，亚洲及大洋洲比较内分泌学会（AOSCE）第七届大会

• 发表时间: 2012
• 作者: Otoki Y.;Itoh N.;Treen N.;Marquez E.;Osada M
• 通讯作者: Osada M

ホタテガイGnRH の性転換と精子形成への関与

扇贝 GnRH 参与性别变化和精子发生

• 发表时间: 2013
• 作者: 大内仁志;伊藤直樹;高橋計介;尾定誠
• 通讯作者: 尾定誠

ホタテガイGnRHの性転換と精子形成への関与

扇贝 GnRH 参与性别变化和精子发生

• 发表时间: 2013
• 作者: 大内仁志;他3名
• 通讯作者: 他3名