Osteochondral regeneration with scaffold free 3D-structure of swine adipose tissue-derived mesenchymal stem cells.

本研究旨在利用脂肪组织源性间充质干细胞（AT-MSCs）的三维结构研究关节软骨和软骨下骨的再生。在股骨滑车沟骨软骨缺损处植入约760个球体，每个球体含有50,000个自体at - mscs。在术后的CT图像中，从骨和种植体之间的边界出现了一个不透射线的区域，并且在种植体中更稳定地向上和向内增加。6个月后，植入物的组织病理学显示，在饱满的纤维软骨下面有活跃的软骨内骨化。12个月后的组织病理学检查显示，不仅减少的纤维软骨与周围正常软骨一样厚，而且还观察到大量软骨下骨。我们认为，植入无支架的AT-MSCs 3D结构将诱导软骨和软骨下骨的原始结构再生。

This study was designed to investigate the regeneration of articular cartilage and subchondral bone using a three-dimensional (3D) construct of adipose tissue-derived mesenchymal stem cells (AT-MSCs). A construct consisting of about 760 spheroids each containing 50,000 autologous AT-MSCs was implanted into an osteochondral defect at the femoral trochlear groove. In CT images after surgery, a radiopaque area emerged from the boundary between the bone and the implant and increased more steadily upward and inward in the implants. Histopathology of the implants after 6 months revealed active endochondral ossification underneath the plump fibrocartilage. Histopathology after 12 months showed that not only was the diminishing fibrocartilage as thick as the surrounding normal cartilage, but massive subchondral bone was also observed. We supposed that implantation of a scaffold-free 3D construct of AT-MSCs will induce regeneration of original structure of cartilage and subchondral bone.