Elucidation of mechanisms governing selectable expression of chloroplast genes and its application to plant improvement

阐明控制叶绿体基因选择性表达的机制及其在植物改良中的应用

• 批准号: 23380205
• 负责人: KOBAYASHI Hirokazu
• 金额: $ 12.81万
• 依托单位: University of Shizuoka
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23380205/
• 关键词: シグナル伝達; 転写制御; タンパク質リン酸化; 葉緑体; 植物

Gene expression in chloroplasts was selectively switched off by the modification of sigma factor 1 (SIG1). We performed genome-wide screening of Arabidopsis cDNA clones by in vitro detection of protein-protein interaction, followed by phosphorylation assay, resulting in identification of some SIG-one protein kinases (SOPKs). Under lighting conditions that preferentially excited PS I, expression profiles of the psaA gene for the reaction center protein of PS I in SOPK-knockout (KO) lines, were consistent with SIG1 being in its unphosphorylated state. Employment of GFP fused with these SOPKs revealed their localization in the cytosol. SIG-one protein phosphatase (SOPH) candidate genes were tried to be identified by similar strategies. This "light switch" was further employed for chloroplast engineering. On the other hand, "gyokuro" green tea enriched with amino acids, is made of tea leaves grown under a light-shaded condition. Such leaves were subjected to transcriptomic analysis.

叶绿体中的基因表达通过sigma因子1（SIG 1）的修饰被选择性地关闭。我们进行了全基因组筛选拟南芥cDNA克隆体外检测蛋白质-蛋白质相互作用，然后通过磷酸化试验，导致一些SIG-1蛋白激酶（SOPKs）的鉴定。在优先激发PS I的光照条件下，SOPK敲除（KO）系中PS I反应中心蛋白的psaA基因的表达谱与SIG 1处于其非磷酸化状态一致。GFP与这些SOPKs融合的就业揭示了它们在胞质溶胶中的定位。采用相似的策略筛选SIG-1蛋白磷酸酶（SOPH）候选基因。这种“光开关”被进一步用于叶绿体工程。另一方面，富含氨基酸的“玉露”绿色茶是由在光照条件下生长的茶叶制成的。对这些叶进行转录组学分析。

项目成果

Enrichment and evaluation of flavonoids in plants treated with LED and engineered with all plant-derived genes

经过 LED 处理并用所有植物源基因改造的植物中黄酮类化合物的富集和评估

• 发表时间: 2012
• 作者: 飯田宰;大西陽向;三柴啓一郎;小泉望;H. Kobayashi
• 通讯作者: H. Kobayashi

Chloroplast biogenesis : Light switch and internal factors for expression of plastid and nuclear genes

叶绿体生物发生：质体和核基因表达的光开关和内部因素

• 发表时间: 2011
• 作者: Matsuzawa T;Fujita Y;Tohda H and Takegawa K;H. Kobayashi
• 通讯作者: H. Kobayashi

Establishment of a tea custom array based on EST data obtained with next-generation sequencing

基于二代测序获得的EST数据建立茶叶定制芯片

• 发表时间: 2013
• 期刊: Proceedings of "The 5th International Conference on O-CHA (Tea) Culture and Science, ICOS 2013"
• 作者: Y. Niwa.;S. Watanabe;T. Ogawa;H. Nagasaki;Y. Nakamura;H. Nishimura;T. Hirayama;E. Kobayashi;Y. Nakamura;H. Kobayashi
• 通讯作者: H. Kobayashi

カスタムマイクロアレイを用いたチャ光制御遺伝子の発現解析

使用定制微阵列对茶光调节基因进行表达分析

• 发表时间: 2014
• 作者: 丹羽康夫;西村秀希;平山隆志;澤田有司;平井優美;長崎英樹;中村保一;小林栄人;渡辺祥子;小川剛史;中村順行;小林裕和
• 通讯作者: 小林裕和

緑体への分化制御因子の同定とその起源／Identification of chloroplast differentiation factor and its origin

叶绿体分化因子的鉴定及其来源

• 发表时间: 2012
• 作者: Sasaki;Midori; Kondo;Yoshinori;丹羽康夫
• 通讯作者: 丹羽康夫