Integrated approach for identification of tumor-suppressive microRNAs and clinical application to miRNA-based therapy for cancers

本研究的目的是鉴定肿瘤抑制microrna （mirna）及其直接靶点，这可能有助于发展基于mirna的癌症治疗的临床应用。实际上，在这项研究中，通过我们基于功能和甲基化筛选的综合方法，确定了三种肿瘤抑制miRNA，一种emt抑制miRNA及其直接靶点。此外，我们在这里证明了双链rna （dsRNA）模拟肿瘤抑制mirna序列的巨大潜力，可以应用于基于mirna的癌症治疗。

The aim of this study is to identify tumor-suppressive microRNAs (miRNAs) and their direct targets, which potentially contribute to the development of clinical application to miRNA-based therapy for cancers. Actually, in this study, three tumor-suppressive miRNAs, one EMT-suppressive miRNA, and their direct targets were identified through our integrated approach using function- and methylation-based screenings. Moreover, we demonstrated here the great potential of double-stranded RNAs (dsRNA) mimicking sequences of tumor-suppressive miRNAs to be applied to miRNA-based therapy for cancers.