Development of therapy for endometriosis.

子宫内膜异位症治疗的发展。

• 批准号: 23390388
• 负责人: SUGIHARA KAZUHIRO
• 金额: $ 12.15万
• 依托单位: Hamamatsu University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23390388/
• 关键词: 創薬; 産婦人科学; 子宮内膜症; DDS型分子標的治療薬

Endometriosis is a common gynecological disease associated with pelvic pain and infertility. Current treatments include oral contraceptives combined with NSAIDs or surgery to remove lesions, all of which provide a temporary but not complete cure. As most endometriosis occurs on organ surfaces facing the peritoneum, we subtracted a phage display library with female mouse peritoneum tissue and selected phage clones by binding to human endometrial epithelial cells. We identify an endometriosis-targeting peptide internalized by cells, designated z13. Proteomics analysis revealed the z13 receptor as the CNGB3. We then linked z13 with an apoptosis inducing peptide and with an endosome-escaping peptide. When these peptides were coadministered into the peritoneum of baboons with endometriosis, cells in lesions selectively underwent apoptosis with no effect on neighboring organs. This study reports a potential therapeutic capable of eliminating endometriosis from the human peritoneum.

子宫内膜异位症是一种常见的妇科疾病，与盆腔疼痛和不孕有关。目前的治疗方法包括口服避孕药联合NSAID或手术切除病变，所有这些都提供了暂时但不完全的治愈。由于大多数子宫内膜异位症发生在面向腹膜的器官表面，我们减去了噬菌体展示库与雌性小鼠腹膜组织和选择的噬菌体克隆结合到人子宫内膜上皮细胞。我们确定了一个由细胞内化的靶向肽，命名为z13。蛋白质组学分析显示z13受体为CNGB3。然后，我们将z13与凋亡诱导肽和内体逃逸肽连接。当这些肽共同管理到狒狒的腹膜子宫内膜异位症，病变细胞选择性地进行细胞凋亡，对邻近器官没有影响。这项研究报告了一种潜在的治疗能够消除子宫内膜异位症从人类腹膜。

项目成果

Tumor targeting by a carbohydrate ligand-mimicking peptide.

• DOI: 10.1007/978-1-62703-465-4_28
• 发表时间: 2013-01-01
• 期刊: Methods in molecular biology (Clifton, N.J.)
• 作者: Hatakeyama, Shingo;Shibata, Toshiaki K;Fukuda, Michiko N
• 通讯作者: Fukuda, Michiko N

Attenuation of fibroblast growth factor signaling by poly- N- acatyllactosamine type glycans implicated to human sperm motility

与人类精子活力相关的聚 N-乙酰基乳糖胺型聚糖减弱成纤维细胞生长因子信号传导

• 发表时间: 2015
• 作者: 臼見 莉沙;藤田 紘一;渋谷 直樹;米満 郁男;大村 進;藤内 祝;小野 卓史;Toshiaki Shibata
• 通讯作者: Toshiaki Shibata

Transient ligation of umbilical vessels elevates placental tissue oxygen index (TOI) values measured by near-infrared spectroscopy (NIRS) in clawn miniature pig animal model.

脐血管瞬时结扎可提高近红外光谱 (NIRS) 在爪型小型猪动物模型中测量的胎盘组织氧指数 (TOI) 值。

• 发表时间: 2012
• 期刊: Clin Exp Obstet Gynecol.
• 作者: Suzukin K;Itoh H;Muramatsu K;Yamazaki K;Nagahashi K;Furuta N;Tamura N;Uchida T;Sugihara K;Sumimoto K;Kanayama N.
• 通讯作者: Kanayama N.

糖鎖をmimicするペプチドを用い腫瘍血管内皮細胞を標的とするドラッグデリバリーシステム

使用模拟糖链的肽靶向肿瘤血管内皮细胞的药物输送系统

• 发表时间: 2013
• 作者: 柴田俊章;杉原一廣;木村たか子;高田佳世子;中村友紀;俵史子;金山尚裕;杉原一廣
• 通讯作者: 杉原一廣

ペプチド創薬

肽药物发现

• 发表时间: 2013
• 作者: 柴田俊章;杉原一廣;木村たか子;高田佳世子;中村友紀;俵史子;金山尚裕;杉原一廣;杉原一廣;杉原一廣;杉原一廣
• 通讯作者: 杉原一廣