Development of a method for simultaneous detection of 4 opportunistic pathogen in mouse

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Opportunistic organisms, which rarely cause infections in immunocompetent mice, can cause infections in immunocompromised mice. Since opportunistic infection may alter research results using immunocompromised mice, health certificate showing the immunocompromised mouse colony is free from specific opportunistic pathogens is important for biomedical researchers.The purpose of this study is development of simple and rapid diagnostic loop-mediated isothermal amplification (LAMP) methods for detecting 4 opportunistic pathogens: Staphylococcus aureus (Sa), mouse hepatitis virus (MHV), murine norovirus (MNV), and Pasteurella pneumotropica (Pp). Reverse transcription-LAMP methods for the detection of MHV and MNV have been developed and published in the Journal of Virological Methods. LAMP methods for the detection of antiseptic- and methicillin-resistant Sa had also been developed. However, LAMP method for the detection of Pp has been under construction.