Application to somatic cell nuclear transfer technology and the development of advanced reproductive technology engineering corresponding to the conservation of mouse genetic resources

利用卵巢中存在的未成熟卵母细胞被认为是未来扩展到辅助生殖医学领域的技术。申请人已经成功地生产了婴儿，考虑使用未成熟卵母细胞的体外受精方法和体外成熟操作的改进，使用近交系小鼠。随着成熟操作培养基的发展，初始卵的基因表达影响氨基酸组分。体细胞储备考虑进一步通过受试小啮齿动物建立成纤维细胞，获得，激活后，我们已经成功地生产克隆胚胎，通过体细胞核移植创建重构胚胎。

Use of immature oocytes present in the ovary is considered as technology leading to expansion into assisted reproductive medical field in the future. The applicant has succeeded in producing infants to consider in vitro fertilization method and improvement of in vitro maturation operation using the immature oocytes, were used inbred mice. It was also acknowledged that advances the development of mature operation medium, gene expression of initial egg affects the amino acid component. The somatic cells stock to consider the establishment of the fibroblast cells by the tested small rodents further, obtained, after activation, we have succeeded in the production of cloned embryos to create a reconstructed embryos by somatic cell nuclear transfer.