Application to somatic cell nuclear transfer technology and the development of advanced reproductive technology engineering corresponding to the conservation of mouse genetic resources

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Use of immature oocytes present in the ovary is considered as technology leading to expansion into assisted reproductive medical field in the future. The applicant has succeeded in producing infants to consider in vitro fertilization method and improvement of in vitro maturation operation using the immature oocytes, were used inbred mice. It was also acknowledged that advances the development of mature operation medium, gene expression of initial egg affects the amino acid component. The somatic cells stock to consider the establishment of the fibroblast cells by the tested small rodents further, obtained, after activation, we have succeeded in the production of cloned embryos to create a reconstructed embryos by somatic cell nuclear transfer.