Prominin-1-GFP knock-in mouse line: A novel source of fluorescent stem cells to monitor the history of the transplanted murine hematopoietic stem cells

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The general purpose of this research program is to develop a novel knock-in mouse model that would generate in vivo-fluorescent hematopoietic stem cells (HSCs) allowing us to monitor the homing, fate and mobilization of transplanted HSCs. To establish such a system, we will use prominin-1, the first member of a new family of pentaspan membrane proteins and a green fluorescence protein (GFP)-based approach. Mouse prominin-1 was initially identified as a marker of neuroepithelial stem cells while its human orthologue (also referred to as CD133) was independently shown to be expressed on hematopoietic stem and progenitor cells. The role of this protein is still unknown. Nevertheless, given that the expression profile of human prominin-1/CD133 mimics that of the murine molecule including expression in several other developmentally early cells, the prominin-1-GFP fusion protein is an ideal plasma membrane marker to monitor in vivo by optical imaging methods the homing of the fluorescent transplanted HSCs, their proliferation and differentiation capacities and, finally their mobilization in murine transplantation models.