Prominin-1-GFP knock-in mouse line: A novel source of fluorescent stem cells to monitor the history of the transplanted murine hematopoietic stem cells

这个研究项目的总体目的是开发一种新型的敲入小鼠模型，该模型可以在体内产生荧光造血干细胞，使我们能够监测移植的造血干细胞的归巢、命运和动员。为了建立这样一个系统，我们将使用重要蛋白-1，一个新的五角叉膜蛋白家族的第一个成员和一种基于绿色荧光蛋白(GFP)的方法。小鼠显著蛋白-1最初被鉴定为神经上皮干细胞的标志，而其人类同源基因(也被称为CD133)被独立地证明在造血干和祖细胞上表达。这种蛋白质的作用尚不清楚。然而，考虑到人重要蛋白-1/CD133的表达谱与小鼠分子相似，包括在其他发育早期细胞中的表达，突出蛋白-1-GFP融合蛋白是一种理想的质膜标记物，可以通过光学成像方法在体内监测荧光移植的HSCs的归巢、增殖和分化能力，最终在小鼠移植模型中动员。

The general purpose of this research program is to develop a novel knock-in mouse model that would generate in vivo-fluorescent hematopoietic stem cells (HSCs) allowing us to monitor the homing, fate and mobilization of transplanted HSCs. To establish such a system, we will use prominin-1, the first member of a new family of pentaspan membrane proteins and a green fluorescence protein (GFP)-based approach. Mouse prominin-1 was initially identified as a marker of neuroepithelial stem cells while its human orthologue (also referred to as CD133) was independently shown to be expressed on hematopoietic stem and progenitor cells. The role of this protein is still unknown. Nevertheless, given that the expression profile of human prominin-1/CD133 mimics that of the murine molecule including expression in several other developmentally early cells, the prominin-1-GFP fusion protein is an ideal plasma membrane marker to monitor in vivo by optical imaging methods the homing of the fluorescent transplanted HSCs, their proliferation and differentiation capacities and, finally their mobilization in murine transplantation models.