CEHC is the active form of non alpha-tocoperol in vivo

维生素E有八种不同的天然存在形式：四种生育酚（Toc）和四种生育三烯酚（Toc-3），其中alha-Toc尤其是已知是保护细胞膜的主要抗氧化剂。维生素E类似物在体内代谢为羧乙基-羟基苯并二氢吡喃（CEHC）并排泄到尿液中。虽然CEHC形式是维生素E的主要代谢物，但CEHC -特别是γ-CEHC-也被报道具有一些生理功能，例如作为利尿钠激素和抗氧化剂。因此，我研究了维生素E及其代谢产物对脂肪细胞诱导的炎症的影响。首先确定了脂肪细胞（3 T3-L1细胞）与巨噬细胞（RAW264.7细胞）的培养方法。接下来，我检查了维生素E对脂肪细胞诱导的炎症的影响。根据这个结果，我发现γ-生育酚不能抑制脂肪细胞的炎症。

Vitamin E has eight different naturally occurring forms: four tocopherols (Toc) and four tocotrienols (Toc-3), among which alha-Toc, in particular, is knowns to be a major antioxidant for protection fo cellular membranes. Vitamin E analogs are metabolized to carboxyethyl-hydroxychroman (CEHC) in vivo and excreted into urine. Although the CEHC form is the main metabolite of vitamin E, CEHC -particularly gamma-CEHC- has also been reported to have some physiological functions, for example as a natriuretic hormone and an antioxidant. Therefore, I investigated the effect of vitamin E and its metabolite on the adipocytes induced inflammation. At first, I determined the culture method of adipocytes (3T3-L1 cells) with macrophages (RAW264.7 cells). Next, I examined the effect of vitamin E on the adipocytes induced inflammation. According to this result, I was shown that gamma-tocotorienol could not suppress the inflammation of adipocytes.