Structural analysis of sphingoglycolipid from Basidiomycota

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In this study, we found out the synthesis of glycosphingolipids(GSL-1) in Maitake and Enokitake, and reported the structure in detail for the first time. The sugar component of GSL-1 wasassigned to be a glucose. LCB and FA fractions were analyzed by GC and GC/MS, and implied that the main components were C20:1 sphingenine for LCB, and C16:0, 2-OH-C16:0 for FA. Taken together, each GSL-1 of Maitake and Enokitake were same structure and defined as a glucosyl (1-1) ceramide. For biological clarification of the GSLs, the glycolipid fraction containing GSL-1 of Maitake and Enokitake were intraperitoneal injection to mouse. Numbers of peritoneal leukocyte were higher in mouse which intraperitoneal injected glycolipid fraction. From the results, the immunostimulatory effect of the glycolipid of Maitake and Enokitake was suggested.