Search for RASSF-interacting molecules using C. elegans

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We screened molecules interacting with C. elegans RASSF homolog, T24F1.3 (we named as rasf-1), by yeast two hybrid system and obtained rab-39 and F11E6.7. We named F11E6.7 as rif-1 because it is phylogenetically close to rif1. Transient expression in HEK293T cells and immunoprecipitation revealed that both mammalian homologs Rab39 and Rif1 bind to RASSF1A. As to the functions of Rab-39 and Rif-1, we demonstrated that Rasf-1 and Rab-39 function in the same signaling pathway in response to oxidative stress and that Rif-1 plays an important role in DNA damage repair as mammalian Rif1.