Clinical significance of Fatty Acid Binding Protein 5 in hepatocellular carcinoma

脂肪酸结合蛋白5在肝细胞癌中的临床意义

• 批准号: 23501293
• 负责人: YOKOO Hideki
• 金额: $ 3.33万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23501293/
• 关键词: 肝細胞癌; バイオマーカー; E-FABP; 発現解析

We analyzed the correlation between the expression of FABP5 and malignant behavior of HCC using HCC tissues and cell lines. A total of 243 HCC samples were subjected to immunohistochemistry, and protein expression of FABP5 in HCC cell lines was assessed by Western blot. The intensity of immunostaining in HCC tissues were divided into a strong staining group and a weak staining group. In the strong group, 5-year overall survival rate was 59.5%, compared with 89.9% in the weak group , and 5-year relapse free survival rate in the strong group was 23.7%, compared with 47.2% in the weak group . In vitro, Western blot showed protein expression of highly invasive cell lines was high, while lowly invasive cell was low. The knockdown of FABP5 in highly invasive cell lines also inhibited cell proliferation, migration and invasiveness significantly. FABP5 was closely related with malignancy, and also behaved as a significant prognostic and recurrence factor for HCC patients.

我们利用肝癌组织和肝癌细胞系分析了FABP 5的表达与肝癌恶性行为的相关性。共243例HCC样本进行免疫组化，并通过Western blot评估HCC细胞系中FABP 5的蛋白表达。肝癌组织免疫染色强度分为强染色组和弱染色组。强组5年总生存率为59.5%，弱组为89.9%;强组5年无复发生存率为23.7%，弱组为47.2%。Western blot显示，高侵袭性细胞株蛋白表达量高，而低侵袭性细胞株蛋白表达量低。在高侵袭性细胞系中敲低FABP 5也显著抑制细胞增殖、迁移和侵袭。FABP 5与肝癌的恶性程度密切相关，是影响肝癌患者预后和复发的重要因素。

项目成果

Utility of FABP5 expression as a recurrence and prognostic factor in hepatocellular carcinoma

FABP5 表达作为肝细胞癌复发和预后因素的效用

• 发表时间: 2013
• 作者: Takanori Ohata;Hideki Yokoo
• 通讯作者: Hideki Yokoo

肝細胞癌における予後再発因子としてのFABP5発現の意義

FABP5表达作为肝细胞癌预后复发因素的意义

• 发表时间: 2013
• 作者: 大畑多嘉宣;横尾英樹
• 通讯作者: 横尾英樹

肝細胞癌におけるFABP5 の新規バイオマーカーとしての有用性

FABP5 作为肝细胞癌新型生物标志物的用途

• 发表时间: 2014
• 作者: 大畑多嘉宣;横尾英樹
• 通讯作者: 横尾英樹

肝細胞癌における予後再発因子としてのFABP5の有用性について

关于 FABP5 作为肝细胞癌预后复发因素的有用性

• 发表时间: 2013
• 作者: 大畑多嘉宣;横尾英樹
• 通讯作者: 横尾英樹

Utility of Fatty Acid Binding Protein 5 expression as a recurrence and prognostic factor in hepatocellular carcinoma

脂肪酸结合蛋白 5 表达作为肝细胞癌复发和预后因素的效用

• 发表时间: 2013
• 作者: Takanori Ohata;Hideki Yokoo
• 通讯作者: Hideki Yokoo