权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Development of versatile method for identification of the target protein using affinity resins with photo-labile linker

开发使用具有光不稳定接头的亲和树脂来鉴定靶蛋白的通用方法

基本信息

批准号：
23510269
负责人：
TANAKA AKITO
金额：
$ 3.41万
依托单位：
Hyogo University of Health Sciences
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2011
资助国家：
日本
起止时间：
2011 至 2013
项目状态：
已结题

项目摘要

We have here develped an effective method for identification of bio-active compound such as drug, natural product, and so on, using affnity resins from lyates. In this method, we applied photo-labile linker to elute the target protein from affinity resins selectively after mixed with lysate from target organ. We used "AquaFirmus", our products, as solid material in this work because this is chemically stable and hydrophilic enough to reduce the non-specific protein absorption.We first fixed target-known bioactive compounds such as FK506 or benzenesulfonamide on the resin through the photo-libile linker with hydrophilic spacers, and successfully eluted the target proteins by irradation on ice for 5 min. We now apply this novel method for identification of target-unknown compounds including developed compounds of pharmaceutical companies.

本文建立了一种利用亲和树脂对药物、天然产物等生物活性物质进行鉴定的有效方法。在该方法中，我们在与靶器官的裂解物混合后，应用光不稳定连接剂从亲和树脂上选择性地洗脱靶蛋白。我们使用我们的产品“AquaFirmus”作为固体材料，因为它具有化学稳定性和足够的亲水性，可以减少非特异性蛋白质的吸收。我们首先通过亲水性间隔基的光自由基连接物将目标已知的生物活性化合物如FK506或苯磺酰胺固定在树脂上，并成功地洗脱的目标蛋白在冰上照射5分钟。我们现在应用这种新的方法来鉴定目标未知化合物，包括开发的化合物的制药公司。