Metapopulation mapping of endagered freshwater fishes for restoration of the ecosystem in traditional irrigation ponds

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Traditional irrigation ponds in SATOYAMA (rural area of Japan) are important habitats for many endangered animals and plants, and many of them are facing a crisis of environmental changes. Although many people have often been working to restore the environments, most of the habitats are isolated from other wetlands, thus freshwater fishes cannot migrate from other habitats by themselves. To restore the fauna of traditional irrigation ponds, we have to re-introduce the freshwater fishes from the other areas.In this study, I try to reconstruct the meta-population structure of endangered fish, Pseudorasbora pumila subsp. by using complete mitochondrial DNA sequences, and compared the phylogeography of the other freshwater fishes in the same area (Tokai region, Japan). The results indicated that the population of P. pumila subsp. was highly diverged among hilly areas, and suggested that the re-introduction should be restricted in a small landscape including the source population.