Molecular sensing using model membranes

使用模型膜进行分子传感

• 批准号: 23560940
• 负责人: VESTERGAARD Catherine
• 金额: $ 2.75万
• 依托单位: Japan Advanced Institute of Science and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23560940/
• 关键词: 免疫センサー; 流動性; 毒性; 神経保護; アミロイド-β; アルツハイマー病; 電気化学; 生体模倣センサー; アミロイドβ; 生体模倣膜; 膜ダイナミクス

We have (i) shown that amyloid beta (Ab) interacts with membranes in a species-dependent and membrane composition manner. They cause membrane transformation, breach and viscosity. These may contribute towards understanding toxicity mechanisms; (ii) studied how oxidation of cholesterol influences membrane dynamics, demonstrating that temperature increase renders oxidized membranes more fluid. We have similar findings using biological cells. The findings are useful for clarifying the impact of cholesterol in Ab-induced toxicity; (iv) oxidized membranes enhance Ab's association with membranes; (v) potential neuro-protective agents interact with membranes in a structure-dependent manner as does their ability to inhibit Ab aggregation. Unraveling the importance of these compounds, at a functional group level opens the key to tailored design of potential bioactive drug candidates. We have developed an immunosensor chip for Ab.

我们 (i) 表明β淀粉样蛋白 (Ab) 以物种依赖性和膜组成方式与膜相互作用。它们会引起膜变形、破裂和粘度。这些可能有助于理解毒性机制； (ii) 研究了胆固醇的氧化如何影响膜动力学，证明温度升高使氧化膜更具流动性。我们使用生物细胞也得到了类似的发现。这些发现有助于阐明胆固醇在抗体引起的毒性中的影响； (iv) 氧化膜增强抗体与膜的结合； (v) 潜在的神经保护剂以结构依赖性方式与膜相互作用，其抑制抗体聚集的能力也是如此。在功能组水平上揭示这些化合物的重要性打开了潜在生物活性候选药物定制设计的关键。我们开发了抗体免疫传感器芯片。

项目成果

Thermo-induced dynamics of membranes containing cholesterol derivatives

含有胆固醇衍生物的膜的热诱导动力学

• 发表时间: 2012
• 期刊: Lipids
• 影响因子: 1.9
• 作者: Yoda;T.;Vestergaard;C.M.*;Hamada;T.;Le P.T.;Takagi;M.
• 通讯作者: M.

Functionalized multi-walled carbon nanotubes as supporting matrix for enhanced ethanol oxidation on Pt-based catalysts

• DOI: 10.1016/j.elecom.2011.03.041
• 发表时间: 2011-07
• 期刊: Electrochemistry Communications
• 影响因子: 5.4
• 作者: Le Ngoc Quynh Hoa;Mun'delanji C. Vestergaard;H. Yoshikawa;M. Saito;E. Tamiya

A rapid gel electrophoretic chip for serum cholesterol determination.

• DOI: 10.1039/c0an00992j
• 发表时间: 2011-04
• 期刊: The Analyst
• 作者: Kenta Kaminikado;Ryuzoh Ikeda;K. Idegami;N. Nagatani;Mun'delanji C. Vestergaard;M. Saito;E. Tamiya

Gold nano-structure LSPR-based biosensor for biomedical diagnosis, In Springer series on Chemical Sensors and Biosensor, "Applications of Nanomaterials in Sensors and Diagnostics"

用于生物医学诊断的基于金纳米结构LSPR的生物传感器，在Springer化学传感器和生物传感器系列中，“纳米材料在传感器和诊断中的应用”

• 作者: Vestergaard M. C.;Saito M.;Yoshikawa H.;Tamiya E.
• 通讯作者: Tamiya E.

Selective localization of Alzheimer's amyloid beta in membrane lateral compartments

• DOI: 10.1039/c2sm07185a
• 发表时间: 2012-01-01
• 期刊: SOFT MATTER
• 影响因子: 3.4
• 作者: Morita, Masamune;Hamada, Tsutomu;Takagi, Masahiro
• 通讯作者: Takagi, Masahiro