Discovery of novel enantioselective imine reductases and efficient enzyme synthesis of optically active amines

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Chiral amines are useful compounds for the synthesis of pharmaceuticals. In an efficient chemical synthesis of optically active amine, asymmetric reduction of imine has been well studied. However, enzymatic reduction of synthetic imine have not been reported yet. We therefore surveyed imine reductase from soil samples , and first found R-and S-imine reductases. After purification and characterization of the enzymes, gene analysis and preparation of recombinant cells were performed. We efficiently synthesized optically active amines with high optical purity using their imine reductases.