Study of relationship between culture microenvironments of embryoid bodies and differentiation properties of stem cells

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In this study, we investigated that the effects of culture microenvironments of embryoid bodies (EBs) on the stem cell differentiation. To control the culture microenvironments of EBs, we designed two kinds of microchips, microwell and micropattern chips by microfabrication and surface modification techniques. In the microwell chip culture, growth and differentiation of EBs were influenced by the microwell diameter, distance between the microwells, and microwell numbers. In the micropattern chip culture, growth and differentiation of EBs were influenced by the presence of neighboring EBs when the separation distance was less than 500 micron. These results indicated that the designs of culture microenvironments of EBs are important factors for regulation of stem cell properties.