The study on lipid modification of extrinsic proteins and thermotorelance of photosynthesis

以集胞藻（Synechocystis sp.PCC 6803）和鱼腥藻（Anabaena sp.PCC 7120）为实验材料，研究了蓝藻光系统Ⅱ复合体（PSII）的耐温机理，着重探讨了脂质和外源蛋白在PSII稳定中的作用。结果发现，至少有一个与PSII的还原侧相关的磷脂酰甘油分子参与PSII的稳定化，不仅影响PSII的还原侧，而且影响PSII的氧化侧，即，外源蛋白质与放氧复合物的功能性结合。我们制作了一个鱼腥藻突变体，其中一个组氨酸标签连接到PSII的一个亚基，以允许从细胞中快速分离高度纯化的PSII复合物。

Mechanism of thermotorelance of cyanobacterial photosystem II complex (PSII) has been investigated using Synechocystis sp. PCC 6803 and Anabaena sp. PCC 7120 focusing on the role of lipids and extrinsic proteins in stabilization of PSII. It was found that at least one phosphatidylglycerol molecule associated with the reducing side of PSII is involved in the stabilization of PSII through affecting not only the reducing-side but also the oxidizing-side of PSII, namely, functional binding of extrinsic proteins to oxygen-evolving complex. We made an Anabaena mutant in which a histidine-tag was attached to one of subunits of PSII to allow the isolation of highly purified PSII complex rapidly from the cells.