DNA analysis for choromosome size changing in plant

植物染色体尺寸变化的 DNA 分析

• 批准号: 23570122
• 负责人: HOSHI Yoshikazu
• 金额: $ 3.58万
• 依托单位: Tokai University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23570122/
• 关键词: 種分化; 染色体; 反復配列

Angiosperms show unusually wide range of genomes size with nearly 2,000-fold. The largest DNA amount measured is found in Fritillaria species (Liliaceae) with about 130 pg (130,000 Mbp) in the genome, possessing gigantic chromosomes with 10 to 20 µm. In contrast, the smallest genome is recently found in two carnivorous plant species of Genlisea (Lentibulariaceae) with just 0.06 pg (63 Mbp). The plant groups with wide genome ranges are restricted to some large families such as Asteraceae, Fabaceae, Liliaceae, Orchidaceae and Poaceae. Whereas sundew carnivorous plant group, the Droseraceae and its closely related family Drosophyllaceae, show unexpectedly high genome size variation with tiny to giant chromosomes. New insight was obtained after analyzing large (L-type) and small (S-type) chromosome sets in the genome composition in Aster (Asteraceae) and Drosera (Droseraceae).

被子植物的基因组显示出异常广泛的范围，大小接近2,000倍。在贝母属物种（百合科）中发现了最大的DNA量，基因组中约有130 pg（130，000 Mbp），拥有10至20 µm的巨大染色体。相比之下，最近在Genlisea（Lentibulariaceae）的两种食肉植物物种中发现了最小的基因组，仅为0.06 pg（63 Mbp）。基因组分布广泛的植物类群仅限于菊科、豆科、百合科、兰科和禾本科等大科。而茅膏菜属食肉植物类群，即茅膏菜科及其密切相关的茅膏菜科，显示出出乎意料的高基因组大小变异，具有微小到巨大的染色体。在分析了紫菀属（菊科）和茅膏菜属（茅膏菜科）基因组组成中的大（L型）和小（S型）染色体组后，获得了新的见解。

项目成果

RAPD profiling of Drosera spatulata and its related species

茅膏菜及其相关物种的 RAPD 分析

• 发表时间: 2012
• 作者: Inagaki;A.;Shirakawa;J. and Hoshi;Y.
• 通讯作者: Y.

A chromosome study of two centromere differentiating Drosera species, D. arcturi and D. regia

两种着丝粒分化茅膏菜物种 D. arcturi 和 D. regia 的染色体研究

• 发表时间: 2011
• 期刊: Caryologia
• 影响因子: 2.1
• 作者: Shirakawa;J.;Nagano;K. and *Hoshi;Y.
• 通讯作者: Y.

RAPD fragment FISH analysis of Drosera spatulata complex (Droseraceae)

茅膏菜复合体（茅膏菜科）的 RAPD 片段 FISH 分析

• 发表时间: 2011
• 作者: Shirakawa;J.;Hoshi;Y.;Nagano;K. and Kondo;K.
• 通讯作者: K.

Polyploid genome structure of Drosera spatulata complex (Droseraceae)

茅膏菜复合体（茅膏菜科）的多倍体基因组结构

• 发表时间: 2012
• 期刊: Cytologia
• 影响因子: 1
• 作者: Shirakawa;J.;Nagano;K. and Hoshi;Y.
• 通讯作者: Y.

DNA polymorphic analysis of Drosera spatulata complex (Droseraceae)

茅膏菜科 (Droseraceae) 茅膏菜复合体 DNA 多态性分析

• 发表时间: 2013
• 作者: Tungkajiwangkoon;S.;Inagaki;A.;Shirakawa;J. and Hoshi;Y.
• 通讯作者: Y.