Analysis of structural and functional changes in immunoglobulins during the maturation of the immune response

免疫反应成熟过程中免疫球蛋白结构和功能变化的分析

• 批准号: 23570169
• 负责人: SHIMIZU Takeyuki
• 金额: $ 3.49万
• 依托单位: Kochi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23570169/
• 关键词: 免疫学; 抗体; 免疫応答成熟; 抗原親和性; 免疫記憶; 体細胞突然変異

By analyzing sequences of the IgM genes expressed in plasma cells which were isolated from secondary immunized mice, we found many VH genes which were rarely found in IgM repertoire of the primary immune response or in IgG repertoire. These IgM antibodies showed very low affinity to antigen, although they activated complement pathway and signaling pathway when they recognized antigens. These findings indicated that in the secondary immune response to T cell-dependent antigen, not only high affinity IgG antibodies but also low affinity IgM antibodies are produced.

通过对二次免疫小鼠浆细胞中IgM基因的序列分析，发现了许多在初次免疫应答的IgM库或IgG库中罕见的VH基因。这些IgM抗体对抗原的亲和力很低，尽管它们在识别抗原时激活补体途径和信号传导途径。这些发现表明，在对T细胞依赖性抗原的二次免疫应答中，不仅产生高亲和力IgG抗体，而且产生低亲和力IgM抗体。

项目成果

B Lymphocyte Cadherin, BILLcadherin/cadherin-17, Contributes to the Survival of Memory B Cells

B 淋巴细胞钙粘蛋白 (BILLcadherin/cadherin-17) 有助于记忆 B 细胞的存活

• 发表时间: 2013
• 作者: 船越 舟一;清水健之;大西一夫
• 通讯作者: 大西一夫

Detection of anti-(4-hydroxy-3-nitrophenyl)acetyl antibody secreting cells by cellular affinity matrix technology

细胞亲和矩阵技术检测抗(4-羟基-3-硝基苯基)乙酰抗体分泌细胞

• 发表时间: 2013
• 作者: 清水健之;宇高恵子;東隆親
• 通讯作者: 東隆親

Ganglioside合成酵素欠損マウスにおけるCD8, CD4 T細胞のin vivo機能の解析

神经节苷脂合酶缺陷小鼠体内 CD8 和 CD4 T 细胞的功能分析

• 发表时间: 2012
• 作者: 佐野ほずみ;清水健之;福田絵美;田中健一;宇高恵子.
• 通讯作者: 宇高恵子.

Detecti on of anti-(4-hydroxy-3-nitrophenyl) acetyl antibody secreting cells by ce llular affinity matrix technology

细胞亲和基质技术检测抗(4-羟基-3-硝基苯基)乙酰抗体分泌细胞

• 发表时间: 2013
• 作者: 清水健之;宇高恵子;東隆親.
• 通讯作者: 東隆親.

Repertoire analysis of anti- (4-hyd roxy-3-nitrophenyl)acetyl IgM antibod ies produced in plasma cells

浆细胞中产生的抗（4-羟基-3-硝基苯基）乙酰基 IgM 抗体的谱分析

• 发表时间: 2012
• 作者: 清水健之;宇高恵子;片岡佐誉;東隆親.
• 通讯作者: 東隆親.