Elucidation of polarity mechanism in migrating cells and analysis of molecular dynamics of phospholipase D on the cell membrane

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Cell migration plays a major role in a variety of normal biological processes and metastasis of cancer cells. It is known that cell migration is controlled by several cell events (e.g., regulation of actin-cytoskeleton, substrate adhesion, membrane traffic, and cell polarity). As a consequence, the system regulating cell migration is very complicated, requiring coordinated spatiotemporal control of these cell events during the migration process. Hitherto, we screened genes regulating cell migration by using our screening system, and PLD and 32 kinase-related genes were identified. In this study, I investigated the single molecule dynamics and activation of PLD on cell membrane by visualization of PLD and its products, phosphatidic acid. Furthermore, I performed pathway analysis among identified gene products to construct a comprehensive signal network of cell migration.