Analysis of the niche for mouse germ cell specification

小鼠生殖细胞规范的利基分析

• 批准号: 23570258
• 负责人: TANAKA Satomi
• 金额: $ 3.41万
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23570258/
• 关键词: 生殖細胞; マウス; WNTシグナル; 発生生物学; BMPシグナル

In the mouse, the formation of primordial germ cells (PGCs) is influenced by BMP4 and WNT3 activity. Embryos lacking Dullard activity fail to form PGCs. We found mutations in Dullard had no detectable effect on BMP4. Results of the genetic study showed that loss of Dullard has no discernible effect on the expression of Bmp4 and Smads, and does not affect the dose-dependent inductive activity of BMP4 on PGC formation. Instead, Dullard functions as an agonist that modulates WNT signalling activity to facilitate the formation of PGC precursors. Loss of Dullard function was accompanied by down-regulation of WNT/beta-catenin signalling activity and a reduction in the level of Dishevelled 2 (Dvl2). Therefore, Dullard may play a role in the fine-tuning of WNT signaling activity, which is crucial for the specification of the germ cell lineage.

在小鼠中，原始生殖细胞（PGCs）的形成受BMP4和WNT3活性的影响。缺少Dullard活性的胚胎不能形成PGCs。我们发现Dullard突变对BMP4没有可检测到的影响。遗传研究结果显示，Dullard缺失对Bmp4和Smads的表达没有明显影响，也不影响Bmp4诱导PGC形成的剂量依赖性活性。相反，Dullard作为一种激动剂，调节WNT信号活动，促进PGC前体的形成。Dullard功能的丧失伴随着WNT/ β -连环蛋白信号活性的下调和disheveled 2 （Dvl2）水平的降低。因此，Dullard可能在WNT信号活动的微调中发挥作用，这对生殖细胞谱系的规范至关重要。

项目成果

Six1 and Six4 homeodomain proteins act downstream to BMP signal in mouse primordial germ cell formation

Six1 和 Six4 同源域蛋白在小鼠原始生殖细胞形成中作用于 BMP 信号下游

• 发表时间: 2012
• 作者: S.S. Tanaka;et al.
• 通讯作者: et al.

Stage-specific Importin13 activity influences meiotic differentiation of germ cells in the mouse

阶段特异性 Importin13 活性影响小鼠生殖细胞的减数分裂分化

• 发表时间: 2011
• 作者: S.S. Tanaka;et al.
• 通讯作者: et al.

Dullard controls primordial germ cell formation by regulating canonical WNT signalling activity in the mouse embryo.

Dullard 通过调节小鼠胚胎中典型的 WNT 信号活性来控制原始生殖细胞的形成。

• 发表时间: 2011
• 作者: Y. L. Yamaguchi;et al.;Satomi S. Tanaka
• 通讯作者: Satomi S. Tanaka

Homeoproteins Six1 and Six4 Regulate Male Sex Determination and Mouse Gonadal Development

• DOI: 10.1016/j.devcel.2013.06.018
• 发表时间: 2013-08-26
• 期刊: DEVELOPMENTAL CELL
• 影响因子: 11.8
• 作者: Fujimoto, Yuka;Tanaka, Satomi S.;Nishinakamura, Ryuichi
• 通讯作者: Nishinakamura, Ryuichi

Sall4 is required for suppression of the somatic gene program during mouse germ cell specification through the epigenetic modification

在小鼠生殖细胞特化过程中，通过表观遗传修饰抑制体细胞基因程序需要 Sall4

• 发表时间: 2011
• 作者: Y. L. Yamaguchi;et al.
• 通讯作者: et al.