Development of a new cultivar production system using transgenic plants in ornamental plants

在观赏植物中使用转基因植物开发新品种生产系统

• 批准号: 23580037
• 负责人: NAKANO MASARU
• 金额: $ 3.49万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23580037/
• 关键词: 花き園芸植物; ホトトギス類; 形質転換体; 遠縁種間雑種; 胚救出; ソマクローナル変異; 染色体倍加; 遠縁種間交雑

White-flowered transgenic plants resulted from RNAi suppression of the CHS gene and dwarf transgenic plants resulted from overexpression of the GA2ox gene were selected as breeding materials through characterization of various transgenic plants of Tricyrtis spp. Interspecific hybrids were produced via ovule culture when dwarf transgenic plants were used as parents. Some of these hybrids showed not only dwarf phenotypes due to the transgene but also novel flower characteristics compared with the parents, indicating the possibility of interspecific hybridization using transgenic plants in the improvement programs of ornamental plants. On the other hand, induction of somaclonal variants and chromosome doubling were also examined by using embryogenic callus cultures of transgenic plants. Detailed characterization of callus culture-derived plants are now in progress.

通过对Tricyrtis spp.各种转基因植株的鉴定，筛选出CHS基因RNAi抑制产生的白花转基因植株和GA2ox基因过表达产生的矮秆转基因植株作为育种材料。以矮秆转基因植株为亲本，通过胚珠培养产生种间杂种。其中一些杂交种不仅表现出转基因植株的矮化表型，而且与亲本相比还表现出新的花型特征，这表明利用转基因植株进行种间杂交在观赏植物改良中是可能的。另一方面，利用转基因植株的胚性愈伤组织培养，还检测了体细胞无性系变异的诱导和染色体加倍。愈伤组织培养衍生植物的详细特征目前正在进行中。

项目成果

Interspecific hybridization between Tricyrtis flava and T. formosana via ovule culture

黄蓟与台湾红蓟的胚珠培养种间杂交

• 发表时间: 2012
• 作者: Haruka TASAKI;Hiroki TAKAGI;Hanae OTSUBO;Chika HATAKEYAMA;Yusaku SAITO;Eriko OKA;Junji AMANO;Yusuke WATANABE;Dong-Sheng HAN;Toshinari GODO and Masaru NAKANO
• 通讯作者: Toshinari GODO and Masaru NAKANO

胚珠培養によるキバナノホトトギスとタイワンホトトギス間の種間雑種の作出

通过胚珠培养培育日本猕猴桃和中华猕猴桃种间杂种

• 发表时间: 2012
• 作者: 石部万葉;田崎晴香;高木宏樹;韓東生;神戸敏成;中野優
• 通讯作者: 中野優

Overexpression of the gibberellin 20-oxidase or gibberellin 3-oxidase gene from Torenia fournieri affects plant morphology in transgenic Tricyrtis sp

蓝猪耳赤霉素 20-氧化酶或赤霉素 3-氧化酶基因的过度表达影响转基因 Tricyrtis sp 的植物形态

• 发表时间: 2014
• 期刊: Acta Horticulturae
• 作者: Masahiro Otani;Shuhei Meguro;Haruka Gondaira;Megumi Hayashi;Misaki Saito;Dong-Sheng Han;Masaru Nakano
• 通讯作者: Masaru Nakano

Horticultural characterization of a tetraploid transgenic plant of Tricyrtis sp. overexpressing the gibberellin 2-oxidase gene from Torenia fournieri

Tricyrtis sp.四倍体转基因植物的园艺特性。

• 发表时间: 2013
• 作者: Masaru Nakano;Mitsuyo Ishibe;Masahiro Otani;Shuhei Meguro;Haruka Gondaira;Megumi Hayashi;Misaki Saito;Dong-Sheng Han;Shiro Mori;Tomoya Niki;Takaaki Nishijima;Masaji Koshioka
• 通讯作者: Masaji Koshioka

Characterization of transgenic Agapanthus praecox spp. orientalis plants ectopically expressing the class B MADS-box genes

转基因百子莲属的表征。

• 发表时间: 2012
• 作者: Nao OKUZUMI;Masahiro OTANI;Hanae OTSUBO;Shuhei MEGURO;Yoshihiro HARA;Hiroto UMEHARA;Mika IGARASHI;Dong-Sheng HAN;Yoichiro HOSHINO;Akira KANNO and Masaru NAKANO
• 通讯作者: Akira KANNO and Masaru NAKANO