Isolation of floral organ morphogenetic genes and elucidation of their involvement in double pistil formation in sweet cherry

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In this study, we isolated the floral organ morphogenetic genes of sweet cherry, and investigated the relationship between their expression level and double pistil formation and ovule development under various temperature conditions and cultivars. The class B MADS box genes, PaTM6 and PaPI, the class C genes, PaAG and PaSHP, and the class D gene, PaSTK, were isolated, and their expression in each flower organ was analyzed. Though there was no clear trend through the whole experiments, it was suggested that the increased expression level of the PaAG gene might be responsible for double pistil formation due to high temperature. On the other hand, it was indicated that the low expression level of the PaSTK gene, might be involved in early ovule degeneration due to gibberellin.