Phytotoxin-like proteins produced by Fusarium oxysporum f. sp. cepae

尖孢镰刀菌Fusarium oxporum f.sp.Cepae(FOC)分泌蛋白质(NIP8和NIP8-S)，当真菌用来自宿主根的抗真菌化合物处理时，这些蛋白质可以引起悬浮培养的宿主细胞的坏死。本研究旨在阐明这些蛋白在FOC致病中的作用。NIP8是疏水素的降解蛋白片段，与FOC的致病力无关。NIP8-S是一种功能未知的碱性蛋白(等电点为9.4)，含有三个推导的二硫键。由大肠杆菌产生的重组NIP8-S在寄主植物上诱导枯萎，表明NIP8-S在引起枯萎病的症状中起作用。

Fusarium oxysporum f. sp. cepae (FOC) secretes proteins (NIP8 and NIP8-S) that can induce necrosis of the suspension-cultured host cells when the fungus is treated with antifungal compounds from the host roots. The present study was performed to clarify the roles of these proteins in the pathogenicity of FOC. NIP8 was proved to be a degraded protein fragment of hydrophobin and unrelated to the pathogenicity of FOC. NIP8-S was a basic protein (pI=9.4) of unknown function with three deduced disulfide bridges. Recombinant NIP8-S produced by Escherichia coli induced wilting on the host plants, suggesting that NIP8-S plays a role for causing the symptoms of Fusarium wilt.