Search on new function of cell-cycle-inhibitors in higher plant.

为了寻找细胞周期抑制剂在高等植物中的新功能，本文以拟南芥为研究对象，研究了kip相关蛋白（KRPs）。在应用前，我用酵母双杂交系统分离了许多atkrp结合蛋白候选物。在这项研究中，我检查了AtKRP与候选药物之间的体外和体内相互作用。至少，其中一个候选物可以用下拉法沉淀AtKRP。候选人申请用酵母-双杂交检测系统确定与AtKRP的结合区域，并确定了该区域。另一个候选基因可能在拟南芥培养的t87细胞中与AtKRP结合。制备了拟南芥AtKRP过表达突变体。为突变体建立了两条同源系。

To search on new function of cell-cycle-inhibitor in higher plant,I focused Arabidopsis KRPs (Kip-related proteins).I isolated many candidates for AtKRP-binding proteins by Yeast two hybrid system before the application. In this study, I checked in vitro and in vivo interaction between AtKRP and the candidates. At least, one of candidate could sedimente with AtKRP by using pull-down assay.The candidate applied to define the binding area with AtKRP by usig yeast-two-hybrid assay system, and the area was defined. Another candidate(s) might be bound with AtKRP in the Arabidopsis culture T87-cells. I also prepared overexpressing-mutant for AtKRP in Arabidopsis plant. Two homo-lines were established for the mutants.