Search on new function of cell-cycle-inhibitors in higher plant.

为了探索细胞周期抑制因子在高等植物中的新功能，本研究以拟南芥Kip相关蛋白（Arabidopsis KRPs）为研究对象，利用酵母双杂交系统分离了多种AtKRPs结合蛋白，并在此基础上进行了初步的应用研究。在这项研究中，我检查了体外和体内的相互作用AtKRP和候选人。用pull-down法确定至少一个候选人与AtKRP的结合面积，用酵母双杂交系统确定结合面积。在拟南芥培养T87-细胞中，另一个候选物可能与AtKRP结合。本研究还制备了AtKRP在拟南芥中的过表达突变体。建立了两个突变体的同源系。

To search on new function of cell-cycle-inhibitor in higher plant,I focused Arabidopsis KRPs (Kip-related proteins).I isolated many candidates for AtKRP-binding proteins by Yeast two hybrid system before the application. In this study, I checked in vitro and in vivo interaction between AtKRP and the candidates. At least, one of candidate could sedimente with AtKRP by using pull-down assay.The candidate applied to define the binding area with AtKRP by usig yeast-two-hybrid assay system, and the area was defined. Another candidate(s) might be bound with AtKRP in the Arabidopsis culture T87-cells. I also prepared overexpressing-mutant for AtKRP in Arabidopsis plant. Two homo-lines were established for the mutants.