The function of peptidylarginine deiminase expressed in the retina of chicken

鸡视网膜表达的肽基精氨酸脱亚胺酶的功能

• 批准号: 23580128
• 负责人: TAKAHARA Hidenari
• 金额: $ 3.41万
• 依托单位: Ibaraki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23580128/
• 关键词: タンパク質アルギニン脱イミノ酵素; ニワトリ; 網膜; 視細胞; 双極細胞; 水平細胞; 鳥類アデノ随伴ウイルス; 過剰発現; siRNA; PAD; chicken; retina; ニワトリ網膜; グリセルアルデヒド３リン酸デヒドロゲナーゼ

Peptidylarginine deiminase is a post-translational modification enzyme that catalyzes the conversion of protein-bound arginine to citrulline in a calcium ion dependent manner. Although PADI genes are widely conserved among vertebrates, their function in the chicken is poorly understood. We cloned and sequenced three chicken PADI cDNAs and analyzed the expression of their proteins in various tissues. Immunoblotting analysis showed that chicken PAD1 and PAD3 were present in cells of several central neuron system tissues including the retina. The chicken PAD1 and PAD3 recombinant proteins required calcium ions as an essential cofactor for their catalytic activity. The two recombinant proteins showed similar substrate specificities toward synthetic arginine derivatives. We conclude that chicken PAD1 and PAD3 might play specific roles in the nervous system, but that chicken PAD2 might not be functional under normal physiological conditions.

肽基精氨酸脱亚胺酶是一种翻译后修饰酶，以钙离子依赖性方式催化蛋白结合精氨酸转化为瓜氨酸。虽然PADI基因在脊椎动物中广泛保守，但其在鸡中的功能知之甚少。我们克隆了三个鸡PADI cDNA并进行了序列测定，分析了它们在不同组织中的蛋白表达。免疫印迹分析表明，鸡PAD 1和PAD 3存在于包括视网膜在内的几种中枢神经系统组织的细胞中。鸡PAD1和PAD3重组蛋白需要钙离子作为其催化活性的必要辅因子。这两种重组蛋白对合成的精氨酸衍生物表现出相似的底物特异性。我们的结论是，鸡PAD 1和PAD 3可能在神经系统中发挥特定的作用，但鸡PAD 2可能在正常生理条件下没有功能。

项目成果

Three Isozymes of Peptidylarginine deiminases in Chicken: Molecular cloning, characterization, and tissue deistribution

鸡肉中肽基精氨酸脱亚胺酶的三种同工酶：分子克隆、表征和组织分布

• 作者: Ａｋｉｒａ Ｓｈｉｍｉｚｕ，Ｔｏｓｈｉo Kojima;Hidenari Takahara
• 通讯作者: Hidenari Takahara

Ｃｈｉｃｋｅｎ ｐｅｐｔｉｄｙｌａｒｇｉｎｉｎｅ ｄｅｉｍｉｎａｓｅ ｔｙｐｅ Ｉ ａｎｄ ＩＩＩ ａｒｅ ｃｏｎｓｔｉｔｕｔｉｖｅｌｙ ｅｘｐｒｅｓｓｅｄ ｉｎ ｔｈｅ ｒｅｔｉｎａｌ ｎｅｕｒｏｎ

鸡肽基精氨酸脱亚胺酶 I 型和 III 型在视网膜神经元中表达

• 作者: 平大輔;中村照也;山系ゆり子;古川憲治;藤井隆夫;友常久実子,春日和,小林正之,池田治生,石川淳,小嶋郁夫;Ａｋｉｒａ Ｓｈｉｍｉｚｕ，Ｔｏｍｏｎｏｒｉ Ｈｏｎｄａ， Ｔｏｓｈｉｏ Ｋｏｊｉｍａ， Ｔｅｔｓｕｙａ Ｋｏｈｓａｋａ， Ｈｉｄｅｎａｒｉ Ｔａｋａｈａｒａ
• 通讯作者: Ａｋｉｒａ Ｓｈｉｍｉｚｕ，Ｔｏｍｏｎｏｒｉ Ｈｏｎｄａ， Ｔｏｓｈｉｏ Ｋｏｊｉｍａ， Ｔｅｔｓｕｙａ Ｋｏｈｓａｋａ， Ｈｉｄｅｎａｒｉ Ｔａｋａｈａｒａ

Three isozymes of peptidylarginine deiminase in the chicken: Molecular

鸡中肽基精氨酸脱亚胺酶的三种同工酶：分子

• 发表时间: 2014
• 期刊: Comparative Biochemistry and Physiology, Part B
• 作者: Akira Shimizu;Kenji Handa ;Tomonori Honda;Naoki Abe;Toshio Kojima;Hidenari Takahara
• 通讯作者: Hidenari Takahara

Three isozymes of peptidylarginine de imiase in the chiken : Molecular cloning、characterization, and tissue distributi on

鸡中肽基精氨酸脱虾酶的三种同工酶：分子克隆、表征和组织分布

• 发表时间: 2014
• 期刊: Comparative Biochemistry and Physio logy, Part B
• 作者: Akira Shimizu;Kenji Handa;Tomonori Honda;Naoki Abe;Toshio Kojima;Hidenar i Takahara
• 通讯作者: Hidenar i Takahara

Chicken peptidylarginine deiminase type I and III are constitutively expre ssed in the retinal neuron

鸡肽基精氨酸脱亚胺酶 I 型和 III 型在视网膜神经元中组成型表达

• 发表时间: 2013
• 作者: Akira Shimizu;Tomonori Honda;Toshio Kojima;Tetsuya Kohsaka;Hidenari Takah ara
• 通讯作者: Hidenari Takah ara