Carbohydrate-recognition and transmembrane ion-channel-formation mechanisms of calcium-dependent lectins

分析了在生物体分子识别系统中起重要作用的钙依赖凝集素的碳水化合物识别机制，设计了一种具有结合专一性的新型人工凝集素。此外，溶血凝集素CEL-III的定点突变和三维结构分析表明，它在靶细胞膜上形成七聚体，并发生广泛的构象变化，形成导致溶血或细胞死亡的跨膜孔。这些结果可能为研究自然界中广泛分布的各种致孔毒素蛋白提供有价值的信息。

Carbohydrate-recognition mechanisms of calcium-dependent lectins, which play important roles in molecular recognition systems in organisms, were analyzed, and a novel artificial lectin with a designed binding-specificity was produced. In addition, site-directed mutational and three-dimensional structural analyses of the hemolytic lectin CEL-III revealed that it forms heptamers in target cell membrane with extensive conformational changes, forming the transmembrane pores that lead to hemolysis or cell death. These results may provide valuable information on various pore-forming toxin proteins widely distributed in the nature.