Elucidation of strigol biosynthesis in Menispermum dauricum

合成了5-脱氧去氧雌酚，建立了高效液相色谱-电喷雾质谱(UPLC-ESI-MS)分析马兜铃内酯的方法。蝙蝠葛素的高产植物蝙蝠葛根培养物中加入5-脱氧雌酚，未检测到5-脱氧雌酚转化为雄酚的现象。这表明蝙蝠葛素不是通过5-脱氧雄酚在蝙蝠葛根中生物合成的。另一方面，合成的内酯类似物GR24被根培养物代谢为7-OH-GR24。然后，用水培方法对高粱高产品种高粱品种高粱进行5-脱氧雌酚的水培试验。Motch，并对其转化为山梨醇进行了研究。5-脱氧去氧雌酚被代谢成山梨醇，并被P450抑制剂抑制。这些结果表明，山梨醇是通过依赖于P450的C-9羟基化从5-脱氧去甲酚生物合成的。

Deuterium-labeled 5-deoxystrigol was synthesized, and the analytical method for strigolactones by UPLC-ESI-MS was established. When the root-cultures of Menispermum dauricum, which are a high producer of strigol, were fed with deuterium-labeled 5-deoxystrigol, the conversion of 5-deoxystrigol to strigol was not detected. This suggests that strigol is not biosynthesized via 5-deoxystrigol in Menispermum dauricum. On the other hand, GR24, a synthetic strigolactone analog, was metabolized to 7-OH-GR24 by the root cultures. Next, deuterium-labeled 5-deoxystrigol was administered to hydroponics of a high sorgomol-producing sorghum cultivar, Sorghum bicolor (L.) Moench, and conversion to sorgomol was investigated. 5-deoxystrigol was metabolized to sorgomol and the conversion was inhibited by a P450 inhibitor. These results suggest that sorgomol is biosynthesized from 5-deoxystrigol via a P450-dependent C-9 hydroxylation.