A study on the mechanisms of appetite regulation in super salmon and its application for aquaculture

鱼类的生长受生长激素（GH）-胰岛素样生长因子（IGF）轴的调节。关于GH转基因鱼体内代谢物的水平和生长增强的机制知之甚少。本研究采用代谢组学方法分析了GH转基因银鲑（Oncorhynchus kisutch）肌肉中的代谢产物，发现转基因银鲑肌肉中糖酵解代谢产物的含量发生了变化。此外，限制摄食还增强了转基因鱼肌肉中某些代谢物水平的变化。然而，在转基因鱼肌肉中发现TCA循环的一些代谢物水平降低。在肌肉中观察到的这些效应与在肝脏中观察到的效应不同。结果表明，GH转基因可以改善碳水化合物的使用，作为与快速生长相关的能量来源。

Growth in fish is regulated by the growth hormone (GH)-insulin-like growth factor (IGF) axis. Little is known concerning the in vivo levels of global metabolites and the mechanism of enhancement of growth in GH transgenic fish. The present study examined the charged metabolites levels in GH transgenic coho salmon (Oncorhynchus kisutch) by metabolomic analysis.The most notable difference found between transgenic and NT fish was that glycolysis metabolite levels changed in the muscle of transgenic fish. In addition, the change in some metabolite levels in the transgenic fish muscle was enhanced by ration-restriction. However, a few metabolites levels of the TCA cycle were found to decrease in the transgenic fish muscle. These effects observed in muscle were different from effects seen in liver. The results suggest that GH transgenesis can improve the use of carbohydrates as a source of energy associated with rapid growth.