A study on the mechanisms of appetite regulation in super salmon and its application for aquaculture

超级鲑鱼食欲调节机制及其在水产养殖中的应用

• 批准号: 23580277
• 负责人: NAKANO TOSHIKI
• 金额: $ 3.49万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23580277/
• 关键词: 農林水産物; 水産学; 栄養学; 組換え食品; バイオテクノロジー; 生理学; 国際情報交流（カナダ）; 国際情報交流

Growth in fish is regulated by the growth hormone (GH)-insulin-like growth factor (IGF) axis. Little is known concerning the in vivo levels of global metabolites and the mechanism of enhancement of growth in GH transgenic fish. The present study examined the charged metabolites levels in GH transgenic coho salmon (Oncorhynchus kisutch) by metabolomic analysis.The most notable difference found between transgenic and NT fish was that glycolysis metabolite levels changed in the muscle of transgenic fish. In addition, the change in some metabolite levels in the transgenic fish muscle was enhanced by ration-restriction. However, a few metabolites levels of the TCA cycle were found to decrease in the transgenic fish muscle. These effects observed in muscle were different from effects seen in liver. The results suggest that GH transgenesis can improve the use of carbohydrates as a source of energy associated with rapid growth.

鱼类的生长受生长激素（GH）-胰岛素样生长因子（IGF）轴的调节。关于GH转基因鱼体内代谢物的水平和生长增强的机制知之甚少。本研究采用代谢组学方法分析了GH转基因银鲑（Oncorhynchus kisutch）肌肉中的代谢产物，发现转基因银鲑肌肉中糖酵解代谢产物的含量发生了变化。此外，限制摄食还增强了转基因鱼肌肉中某些代谢物水平的变化。然而，在转基因鱼肌肉中发现TCA循环的一些代谢物水平降低。在肌肉中观察到的这些效应与在肝脏中观察到的效应不同。结果表明，GH转基因可以改善碳水化合物的使用，作为与快速生长相关的能量来源。

项目成果

Heat shock-induced oxidative stress modulates growth-related gene expressions and redox status in coho salmon

热休克诱导的氧化应激调节银鲑鱼生长相关基因表达和氧化还原状态

• 发表时间: 2011
• 期刊: Comp. Physiol. Biochem.
• 作者: Nakano;T.;Kameda;M.;Shoji;Y.;Yamaguchi;T.;Sato;M.;Afonso;L.;Devlin;R.H.;Iwama;G.K.
• 通讯作者: G.K.

ORAC法によるオキシテトラサイクリン投与ギンザケの抗酸化レベルの評価について、日水秋季大会講要、p.15

使用 ORAC 方法评估土霉素处理的银鲑鱼的抗氧化水平，日本套房秋季会议讲座记录，第 15 页。

• 发表时间: 2013
• 作者: 林聡司;中野俊樹;山口敏康;佐藤実
• 通讯作者: 佐藤実

Natural marine products inhibit formation of 8-chloroguanosine mediated by hypochlorous acid, myeloperoxidase and activated neutrophils

天然海产品抑制次氯酸、髓过氧化物酶和活化中性粒细胞介导的 8-氯鸟苷形成

• 发表时间: 2012
• 期刊: Free Radic. Biol. Med
• 作者: T. Nakano;Y. Shiba;T. Yamaguchi;M. Sato;H. Ohshima
• 通讯作者: H. Ohshima

Rising to the challenge of reconstructing the coastal fisheries environment following the massive tsunami in Japan : the national 10-year"Tohoku Ecosystem-Associated Marine Sciences (TEAMS)"project

应对日本特大海啸后沿海渔业环境重建的挑战：国家十年“东北生态系统相关海洋科学（TEAMS）”项目

• 发表时间: 2013
• 作者: T. Nakano;T. Yamaguchi;M. Sato;R.H. Devlin;T. Nakano
• 通讯作者: T. Nakano

Metabolome profiling of growth hormone transgenic and non-transgenic fish. Seikagaku, 85, 122 (2013)

生长激素转基因和非转基因鱼的代谢组分析。

• 发表时间: 2013
• 作者: T. Nakano;H. Shirakawa;T. Yamaguchi;M. Sato;Y. Suda;M. Saito;H. Maki;T. Soga;G. Yeo;R.H. Devlin
• 通讯作者: R.H. Devlin