Analysis of the mechanism that elaborate an alternative pathogenicity by loss of capsule in Streptococcus suis

猪链球菌荚膜丢失另一种致病机制分析

• 批准号: 23580420
• 负责人: SEKIZAKI TSUTOMU
• 金额: $ 3.49万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23580420/
• 关键词: 人獣共通感染症; 獣医細菌学; 分子遺伝学; レンサ球菌; 病原因子; ブタ; 莢膜; 豚レンサ球菌; 心内膜炎; 遺伝子変異

Streptococcus suis is a bacterial pathogen that causes meningitis or endocarditis in pigs and humans. Capsule, a surface component produced by this bacterium, is an important virulence factor that inhibits phagocytosis of white blood cells. This study showed that acapsular cells of S. suis, which have sometimes been found in swine endocarditis isolates, have spontaneous mutations in capsular polysaccharide synthesis (cps) genes, and that mutations in some cps genes appeared lethal for the bacteria, whereas these lethal effects were relieved by mutations in the other cps genes. Moreover, acapsular cells showed elevated adherence to porcine and human platelets, a major virulence determinant for infective endocarditis, compared with capsular cells and the acapsular cells facilitated the incorporation of capsular cells to the biofilm formed by acapsular cells.

猪链球菌是一种细菌性病原体，可引起猪和人类的脑膜炎或心内膜炎。荚膜是该菌产生的表面成分，是抑制白色血细胞吞噬作用的重要毒力因子。本研究表明，S.有时在猪心内膜炎分离物中发现的SUIS在荚膜多糖合成（CPS）基因中具有自发突变，并且某些CPS基因中的突变对细菌似乎是致命的，而这些致命作用被其它CPS基因中的突变所缓解。此外，无囊细胞表现出较高的粘附猪和人血小板，感染性心内膜炎的主要毒力决定因素，相比，无囊细胞和无囊细胞促进包膜细胞的纳入无囊细胞形成的生物膜。

项目成果

Streptococcus suis: an emerging biothreat

猪链球菌：一种新兴的生物威胁

• 发表时间: 2012
• 期刊: J. Disaster Res.
• 作者: Sekizaki;T.
• 通讯作者: T.

豚レンサ球菌感染症の分子遺伝学的研究

猪链球菌感染的分子遗传学研究

• 发表时间: 2012
• 作者: 野尻彩歌;永松大和;中馬猛久;関崎勉
• 通讯作者: 関崎勉

Lineage and virulence of Streptococcus suis serotype 2 isolates from North America.

• DOI: 10.3201/eid1712.110609
• 发表时间: 2011-12
• 期刊: Emerging infectious diseases
• 影响因子: 11.8
• 作者: Fittipaldi N;Xu J;Lacouture S;Tharavichitkul P;Osaki M;Sekizaki T;Takamatsu D;Gottschalk M
• 通讯作者: Gottschalk M

Streptococcus suis serotyping by a new multiplex PCR

• DOI: 10.1099/jmm.0.069757-0
• 发表时间: 2014-06-01
• 期刊: JOURNAL OF MEDICAL MICROBIOLOGY
• 影响因子: 3
• 作者: Kerdsin, Anusak;Akeda, Yukihiro;Oishi, Kazunori
• 通讯作者: Oishi, Kazunori

Streptococcus suisの再分類に対応するPCR法

猪链球菌重分类的PCR方法

• 发表时间: 2014
• 作者: 石田沙倉;遠矢真理;野本竜平;大澤朗;関崎勉
• 通讯作者: 関崎勉