Development of peptide vaccine using nano-particle adjuvant for naturally occuring tumors in dogs

本研究旨在为犬自然发生的肿瘤建立新的肿瘤疫苗。我们根据肿瘤特异性抗原的表位合成了多肽。将肽与锁孔帽贝血青素偶联，吸附到纳米颗粒（佐剂）表面。所得产物成功地诱导了针对肽的抗体产生，然而，未检测到针对靶细胞的细胞免疫。在接种疫苗的犬中未观察到不良事件，但在淋巴瘤犬中未检测到临床获益（客观肿瘤减少）。新报道了犬TRP-2 cDNA核苷酸序列，构建了TRP-2、melan-A、CD20-GFP融合分子和哺乳动物表达质粒。本研究结果对建立犬肿瘤疫苗有一定的参考价值。

TThe aim of this study is to establish new tumor vaccine for canine naturally occurring tumor. We synthesized peptides in reference to epitope of tumor specific antigens. The peptides were conjugated with keyhole limpet hemocyanin and absorbed to the surface of nano-particle (adjuvant). The resultant products successfully induced antibody production against the peptide, however, cellular immunity against the target cells was not detected. Adverse events were not observed in vaccinated dogs but the clinical benefit (objective tumor reduction) was not detected in a dog with lymphoma. We newly reported canine TRP-2 cDNA nucleotide sequence and constructed TRP-2, melan-A, CD20-GFP fusion molecule, mammalian expression plasmid. The findings obtained in this study may be helpful to establish canine tumor vaccine.