Effect of ketone body to peripheral gd T cell function in dairy cows during the breeding periods

奶牛在哺乳期早期出现疾病的风险增加。此时产奶量增加，但采食量滞后，导致负能量平衡奶牛体内酮体的产生。虽然应该尽量减少高酮血症，以防止对免疫功能和生殖产生不良影响，但本研究探讨了酮体对T细胞功能的影响。在高酮血症奶牛中，IL-17家族作为促炎细胞因子响应免疫系统的入侵，IL-4家族作为抗炎细胞因子响应妊娠，IL-17家族增加。在T细胞比较中，奶牛gd - T细胞强烈表达IL-17 mRNA， CD4+ T细胞强烈表达IL-4 mRNA，因此酮体可能通过对gd - T细胞的激活作用和对CD4+ T细胞的抑制作用诱导了不适当的受孕免疫条件。

Dairy cows are at an increased risk for disorders during early lactation. At this time, there is increasing milk production, but a lag in feed intake, and this condition results in the production of the ketone body in the cows with negative energy balance. Although hyperketonemia should be minimized to prevent undesirable effects on immune function and reproduction, effect of ketone body to T cell function was investigated in this study. Increased IL-17 as a family functions as a proinflammatory cytokine that responds to the invasion of the immune system and decreased IL-4 as an anti-inflammatory cytokine that responds to the gestation were observed in the cows with hyperketonemina. In the comparison of T cell, gd T cell express strongly IL-17 mRNA and CD4+ T cell express strongly IL-4 mRNA in cows, therefore it was possible that ketone bodies induce an inappropriate immune condition for conception by activative effect to gd T cells and suppressive effect to CD4+ T cells.