Development of pH-responsive bio-device by utilizing homomeric to heteromeric coiled-coil conversion

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In this study, we demonstrated that the coiled-coil domains isolated from the yeast cargo receptors Emp46p/47p show pH-dependent conversion of heteromeric to homomeric complexes, and identified the amino acid residues responsible for the regulation of the conversion. Mutations introduced at these residues altered the pH-dependency of the formation of heteromeric complexes. Moreover, using fluorescent protein fusions, the assembly of heteromeric complexes was successfully detected by FRET, which enabled real-time monitoring of the heteromeric complex formation. Thus, we have succeeded in providing strategies for application of the coiled-coil domains of Emp46p/47p to various pH-responsive bio-devices.